Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NR4A2 antibody (Cat#52774, 1:200), overnight at 4°C, followed by conjugation to the SP Kit and DAB staining.
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NR4A2 antibody (Cat#52774, 1:200), overnight at 4°C, followed by conjugation to the SP Kit and DAB staining.
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NR4A2 antibody (Cat#52774, 1:200), overnight at 4°C, followed by conjugation to the SP Kit and DAB staining.
Western blotting analysis using anti-NR4A2 antibody (Cat#52774). Total lysates were loaded and separated by SDS-PAGE. The blot was incubated with anti-NR4A2 antibody (Cat#52774, 1:800) and conjugated goat anti-rabbit secondary antibody respectively.