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Anti-PPP1R9B Mouse Monoclonal Antibody #5283

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Flow cytometric analysis of PPP1R9B expression in HepG2 cells using anti-PPP1R9B antibody (Cat#5283, 1:2,000). Green, isotype control; red, PPP1R9B.

Immunocytochemical staining of HepG2 cells with anti-PPP1R9B antibody (Cat#5283, 1:1,000) . Nuclei were stained blue with DAPI; PPP1R9B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium . Scale bar, 20 μm.

Western blotting analysis using anti-PPP1R9B antibody (Cat#5283). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPP1R9B antibody (Cat#5283, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

PPP1R9B
货号：

5283
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-PPP1R9B Mouse Monoclonal Antibody
Aliases	PPP1R9B; Protein Phosphatase 1 Regulatory Subunit 9B; Neurabin-2; PPP1R6; SPINO; Spn; Protein Phosphatase 1, Regulatory Subunit 9B, Spinophilin; Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 9B; Spinophilin; Neurabin-II; PPP1R9; Protein Phosphatase 1, Regulatory Subunit 9B
Background	Gene Name: PPP1R9B NCBI Gene Entry: 84687 UniProt Entry: Q96SB3
Application Information	Molecular Weight: Predicted, 89 kDa; observed, 130 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB740 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human PPP1R9B
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:400-1:2,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of PPP1R9B expression in HepG2 cells using anti-PPP1R9B antibody (Cat#5283, 1:2,000). Green, isotype control; red, PPP1R9B.
Immunocytochemical staining of HepG2 cells with anti-PPP1R9B antibody (Cat#5283, 1:1,000) . Nuclei were stained blue with DAPI; PPP1R9B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium . Scale bar, 20 μm.
Western blotting analysis using anti-PPP1R9B antibody (Cat#5283). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPP1R9B antibody (Cat#5283, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

PPP1R9B
货号：

5283
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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