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Anti-RECQL Mouse Oligoclonal Antibody #5296

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Flow cytometric analysis of RECQL expression in HepG2 cells using anti-RECQL antibody (Cat#5296, 1:2,000). Green, isotype control; red, RECQL.
Immunocytochemical staining of HepG2 cells with anti-RECQL antibody (Cat#5296, 1:1,000) . Nuclei were stained blue with DAPI; RECQL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-RECQL antibody (Cat#5296). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RECQL antibody (Cat#5296, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    RECQL
  • 货号:
    5296
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-RECQL Mouse Oligoclonal Antibody
Aliases RECQL; RecQ Like Helicase; RecQ1; RecQL1; RecQ Protein-Like (DNA Helicase Q1-Like); DNA Helicase, RecQ-Like Type 1; ATP-Dependent DNA Helicase Q1; DNA-Dependent ATPase Q1; DNA 3'-5' Helicase Q1; RecQ Protein-Like 1; RecQ Helicase-Like; DNA Helicase Q1-Like; EC 5.6.2.4; EC 3.6.1; RECQL1; RECON; RECQ1
Background

Gene Name: RECQL

NCBI Gene Entry: 5965

UniProt Entry: P46063

Application Information

Molecular Weight: Predicted, 73 kDa; observed, 70 kDa

Clonality: Mouse oligoclonal antibody

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen Recombinant protein of human RECQL
Isotype Mouse IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of RECQL expression in HepG2 cells using anti-RECQL antibody (Cat#5296, 1:2,000). Green, isotype control; red, RECQL.
  • Immunocytochemical staining of HepG2 cells with anti-RECQL antibody (Cat#5296, 1:1,000) . Nuclei were stained blue with DAPI; RECQL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-RECQL antibody (Cat#5296). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RECQL antibody (Cat#5296, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of RECQL expression in HepG2 cells using anti-RECQL antibody (Cat#5296, 1:2,000). Green, isotype control; red, RECQL.
Immunocytochemical staining of HepG2 cells with anti-RECQL antibody (Cat#5296, 1:1,000) . Nuclei were stained blue with DAPI; RECQL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-RECQL antibody (Cat#5296). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RECQL antibody (Cat#5296, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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