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KD-Validated Anti-MAPK10 Rabbit Monoclonal Antibody #62328

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Flow cytometric analysis of JNK3 expression in HT-1080 cells using JNK3 antibody (Cat#62328, 1:2,000). Green, isotype control; red, JNK3.

Immunocytochemical staining of HT-1080 cells with anti-JNK3 antibody (Cat#62328, 1:1,000). Nuclei were stained blue with DAPI; JNK3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-JNK3 antibody (Cat#62328). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-JNK3 antibody (Cat#62328, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-JNK3 antibody (Cat#62328). JNK3 expression in wild type (WT) and JNK3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-JNK3 antibody (Cat#62328, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

MAPK10
货号：

62328
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-MAPK10 Rabbit Monoclonal Antibody
Aliases	Mitogen-Activated Protein Kinase 10; JNK3; P54bSAPK; P493F12; PRKM10; Stress-Activated Protein Kinase JNK3; Stress-Activated Protein Kinase 1b; C-Jun N-Terminal Kinase 3; MAP Kinase P49 3F12; MAP Kinase 10; EC 2.7.11.24; SAPK1b; JNK3A; Stress Activated Protein Kinase Beta; JNK3 Alpha Protein Kinase; EC 2.7.11; MAPK 10; SAPK1B
Background	Gene Name: MAPK10 NCBI Gene Entry: 5602 UniProt Entry: P53779
Application Information	Molecular Weight: Predicted, 53 kDa, observed, 53 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB320 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human JNK3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of JNK3 expression in HT-1080 cells using JNK3 antibody (Cat#62328, 1:2,000). Green, isotype control; red, JNK3.
Immunocytochemical staining of HT-1080 cells with anti-JNK3 antibody (Cat#62328, 1:1,000). Nuclei were stained blue with DAPI; JNK3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-JNK3 antibody (Cat#62328). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-JNK3 antibody (Cat#62328, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-JNK3 antibody (Cat#62328). JNK3 expression in wild type (WT) and JNK3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-JNK3 antibody (Cat#62328, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

MAPK10
货号：

62328
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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