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Anti-TBC1D4 Rabbit Monoclonal Antibody #5312

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Flow cytometric analysis of TBC1D4 expression in HepG2 cells using anti-TBC1D4 antibody (Cat#5312, 1:2,000). Green, isotype control; red, TBC1D4.

Western blotting analysis using anti-TBC1D4 antibody (Cat#5312). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TBC1D4 antibody (Cat#5312, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Immunocytochemical staining of HepG2 cells with anti-TBC1D4 antibody (Cat#5312, 1:1,000) . Nuclei were stained blue with DAPI; TBC1D4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

基因名：

TBC1D4
货号：

5312
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-TBC1D4 Rabbit Monoclonal Antibody
Aliases	TBC1D4; TBC1 Domain Family Member 4; AS160; Akt Substrate Of 160 KDa; KIAA0603; DKFZp779C0666; TBC (Tre-2, BUB2, CDC16) Domain-Containing Protein; TBC1 Domain Family, Member 4; NIDDM5
Background	Gene Name: TBC1D4 NCBI Gene Entry: 9882 UniProt Entry: O60343
Application Information	Molecular Weight: Predicted, 147 kDa; observed, 180 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB890 Species Reactivity: Human, mouse Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human TBC1D4
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of TBC1D4 expression in HepG2 cells using anti-TBC1D4 antibody (Cat#5312, 1:2,000). Green, isotype control; red, TBC1D4.
Western blotting analysis using anti-TBC1D4 antibody (Cat#5312). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TBC1D4 antibody (Cat#5312, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with anti-TBC1D4 antibody (Cat#5312, 1:1,000) . Nuclei were stained blue with DAPI; TBC1D4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Citation(0)

基因名：

TBC1D4
货号：

5312
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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