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KD-Validated Anti-SMAD5 Rabbit Monoclonal Antibody #61516

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Flow cytometric analysis of SMAD5 expression in HepG2 cells using SMAD5 antibody (Cat#61516, 1:2,000). Green, isotype control; red, SMAD5.
Immunocytochemical staining of HepG2 cells with SMAD5 antibody (Cat#61516, 1:1,000). Nuclei were stained blue with DAPI; SMAD5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-SMAD5 antibody (Cat#61516). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMAD5 antibody (Cat#61516, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-SMAD5 antibody (Cat#61516). SMAD5 expression in wild type (WT) and SMAD5 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SMAD5 antibody (Cat#61516, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    SMAD5
  • 货号：
    61516
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-SMAD5 Rabbit Monoclonal Antibody
Aliases SMAD Family Member 5; JV5-1; MADH5; Mothers Against Decapentaplegic Homolog; DWFC; MAD, Mothers Against Decapentaplegic Homolog 5 (Drosophila); Mothers Against Decapentaplegic, Drosophila, Homolog Of, 5; SMAD, Mothers Against DPP Homolog 5 (Drosophila) 2; MAD, Mothers Against Decapentaplegic Homolog 5; SMAD, Mothers Against DPP Homolog 5; SMA- And MAD-Related Protein 5; Mothers Against DPP Homolog 5; MAD Homolog 5; SMAD 5; HSmad5; Smad5; Dwfc
Background

Gene Name: SMAD5

NCBI Gene Entry: 4090

UniProt Entry: Q99717
Application Information

Molecular Weight: Predicted, 52 kDa, observed, 52 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2910

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Smad5
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of SMAD5 expression in HepG2 cells using SMAD5 antibody (Cat#61516, 1:2,000). Green, isotype control; red, SMAD5.
  • Immunocytochemical staining of HepG2 cells with SMAD5 antibody (Cat#61516, 1:1,000). Nuclei were stained blue with DAPI; SMAD5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-SMAD5 antibody (Cat#61516). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMAD5 antibody (Cat#61516, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-SMAD5 antibody (Cat#61516). SMAD5 expression in wild type (WT) and SMAD5 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SMAD5 antibody (Cat#61516, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    SMAD5
  • 货号：
    61516
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of SMAD5 expression in HepG2 cells using SMAD5 antibody (Cat#61516, 1:2,000). Green, isotype control; red, SMAD5.
Immunocytochemical staining of HepG2 cells with SMAD5 antibody (Cat#61516, 1:1,000). Nuclei were stained blue with DAPI; SMAD5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-SMAD5 antibody (Cat#61516). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMAD5 antibody (Cat#61516, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-SMAD5 antibody (Cat#61516). SMAD5 expression in wild type (WT) and SMAD5 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SMAD5 antibody (Cat#61516, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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