Flow cytometric analysis of Rho GDP dissociation inhibitor alpha expression in HepG2 cells using anti-Rho GDP dissociation inhibitor alpha antibody (Cat#5335, 1:2,000). Green, isotype control; red, Rho GDP dissociation inhibitor alpha.
Immunocytochemical staining of HepG2 cells with anti-Rho GDP dissociation inhibitor alpha antibody (Cat#5335, 1:1,000). Nuclei were stained blue with DAPI; Rho GDP dissociation inhibitor alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Rho GDP dissociation inhibitor alpha antibody (Cat#5335). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Rho GDP dissociation inhibitor alpha antibody (Cat#5335, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Rho GDP dissociation inhibitor alpha expression in HepG2 cells using anti-Rho GDP dissociation inhibitor alpha antibody (Cat#5335, 1:2,000). Green, isotype control; red, Rho GDP dissociation inhibitor alpha.
Immunocytochemical staining of HepG2 cells with anti-Rho GDP dissociation inhibitor alpha antibody (Cat#5335, 1:1,000). Nuclei were stained blue with DAPI; Rho GDP dissociation inhibitor alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Rho GDP dissociation inhibitor alpha antibody (Cat#5335). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Rho GDP dissociation inhibitor alpha antibody (Cat#5335, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Rho GDP dissociation inhibitor alpha expression in HepG2 cells using anti-Rho GDP dissociation inhibitor alpha antibody (Cat#5335, 1:2,000). Green, isotype control; red, Rho GDP dissociation inhibitor alpha.
Immunocytochemical staining of HepG2 cells with anti-Rho GDP dissociation inhibitor alpha antibody (Cat#5335, 1:1,000). Nuclei were stained blue with DAPI; Rho GDP dissociation inhibitor alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Rho GDP dissociation inhibitor alpha antibody (Cat#5335). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Rho GDP dissociation inhibitor alpha antibody (Cat#5335, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
