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KD-Validated Anti-ASS1 Mouse Monoclonal Antibody #65873

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Flow cytometric analysis of Argininosuccinate synthase 1 expression in HepG2 cells using anti-Argininosuccinate synthase 1 antibody (Cat#65873, 1:2,000). Green, isotype control; red, Argininosuccinate synthase 1.

Western blotting analysis using anti-argininosuccinate synthase 1 antibody (Cat#65873). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-argininosuccinate synthase 1 antibody (Cat#65873, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-argininosuccinate synthase 1 antibody (Cat#65873). Argininosuccinate synthase 1 expression in wild-type (WT) and argininosuccinate synthase 1 (ASS1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-argininosuccinate synthase 1 antibody (Cat#65873, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

ASS1
货号：

65873
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-ASS1 Mouse Monoclonal Antibody
Aliases	ASS1; Argininosuccinate Synthase 1; CTLN1; ASS; Argininosuccinate Synthase; EC 6.3.4.5; Epididymis Secretory Sperm Binding Protein; Argininosuccinate Synthetase 1; Argininosuccinate Synthetase; Citrulline--Aspartate Ligase; Citrulline-Aspartate Ligase
Background	Gene Name: ASS1 NCBI Gene Entry: 445 UniProt Entry: P00966
Application Information	Molecular Weight: Predicted, 47 kDa, observed, 43 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB5600 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human ASS1
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Argininosuccinate synthase 1 expression in HepG2 cells using anti-Argininosuccinate synthase 1 antibody (Cat#65873, 1:2,000). Green, isotype control; red, Argininosuccinate synthase 1.
Western blotting analysis using anti-argininosuccinate synthase 1 antibody (Cat#65873). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-argininosuccinate synthase 1 antibody (Cat#65873, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-argininosuccinate synthase 1 antibody (Cat#65873). Argininosuccinate synthase 1 expression in wild-type (WT) and argininosuccinate synthase 1 (ASS1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-argininosuccinate synthase 1 antibody (Cat#65873, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

ASS1
货号：

65873
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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