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Anti-ETFA Mouse Monoclonal Antibody #5351

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Flow cytometric analysis of Electron transfer flavoprotein subunit alpha expression in HepG2 cells using anti-Electron transfer flavoprotein subunit alpha antibody (Cat#5351, 1:2,000). Green, isotype control; red, Electron transfer flavoprotein subunit alpha.

Immunocytochemical staining of HepG2 cells with anti-Electron transfer flavoprotein subunit alpha antibody (Cat#5351, 1:1,000). Nuclei were stained blue with DAPI; Electron transfer flavoprotein subunit alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: high. Scale bar, 20 μm.

Western blotting analysis using anti-electron transfer flavoprotein subunit alpha antibody (Cat#5351). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-electron transfer flavoprotein subunit alpha antibody (Cat#5351, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

ETFA
货号：

5351
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-ETFA Mouse Monoclonal Antibody
Aliases	ETFA; Electron Transfer Flavoprotein Subunit Alpha; MADD; GA2; EMA; Electron Transfer Flavoprotein Subunit Alpha, Mitochondrial; Multiple Acyl-CoA Dehydrogenase Deficiency; Glutaric Aciduria II; Alpha-ETF; Electron-Transfer-Flavoprotein, Alpha Polypeptide; Electron Transfer Flavoprotein, Alpha Polypeptide; Electron Transfer Flavoprotein Alpha Subunit; Epididymis Secretory Sperm Binding Protein
Background	Gene Name: ETFA NCBI Gene Entry: 2108 UniProt Entry: P13804
Application Information	Molecular Weight: Predicted, 35 kDa; observed, 30 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB1095 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human ETFA
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Electron transfer flavoprotein subunit alpha expression in HepG2 cells using anti-Electron transfer flavoprotein subunit alpha antibody (Cat#5351, 1:2,000). Green, isotype control; red, Electron transfer flavoprotein subunit alpha.
Immunocytochemical staining of HepG2 cells with anti-Electron transfer flavoprotein subunit alpha antibody (Cat#5351, 1:1,000). Nuclei were stained blue with DAPI; Electron transfer flavoprotein subunit alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: high. Scale bar, 20 μm.
Western blotting analysis using anti-electron transfer flavoprotein subunit alpha antibody (Cat#5351). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-electron transfer flavoprotein subunit alpha antibody (Cat#5351, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

ETFA
货号：

5351
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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