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Anti-Clathrin Heavy Chain Rabbit Monoclonal Antibody #5373

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Flow cytometric analysis of Clathrin heavy chain expression in HepG2 cells using anti-Clathrin heavy chain antibody (Cat#5373, 1:2,000). Green, isotype control; red, Clathrin heavy chain.

Immunocytochemical staining of HepG2 cells with anti-Clathrin heavy chain antibody (Cat#5373, 1:1,000). Nuclei were stained blue with DAPI; Clathrin heavy chain was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-clathrin heavy chain antibody (Cat#5373). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-clathrin heavy chain antibody (Cat#5373, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

CLTC
货号：

5373
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-Clathrin Heavy Chain Rabbit Monoclonal Antibody
Aliases	CLTC; Clathrin Heavy Chain; CLTCL; Hc; Clathrin Heavy Chain On Chromosome 17; Clathrin, Heavy Polypeptide-Like 2; Clathrin, Heavy Polypeptide (Hc); Clathrin Heavy Chain 1; CLH-17; Clathrin, Heavy Chain (Hc); Clathrin, Heavy Chain; KIAA0034; CHC17; MRD56; CLH17; CHC
Background	Gene Name: CLTC NCBI Gene Entry: 1213 UniProt Entry: Q00610
Application Information	Molecular Weight: Predicted, 192 kDa; observed, 192 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB1195 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Clathrin heavy chain
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Clathrin heavy chain expression in HepG2 cells using anti-Clathrin heavy chain antibody (Cat#5373, 1:2,000). Green, isotype control; red, Clathrin heavy chain.
Immunocytochemical staining of HepG2 cells with anti-Clathrin heavy chain antibody (Cat#5373, 1:1,000). Nuclei were stained blue with DAPI; Clathrin heavy chain was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-clathrin heavy chain antibody (Cat#5373). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-clathrin heavy chain antibody (Cat#5373, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

CLTC
货号：

5373
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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