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Anti-ATP5C1 Mouse Monoclonal Antibody #5399

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Flow cytometric analysis of ATP synthase F1 subunit gamma expression in HepG2 cells using anti-ATP synthase F1 subunit gamma antibody (Cat#5399, 1:1,000). Green, isotype control; red, ATP synthase F1 subunit gamma.

Immunocytochemical staining of HepG2 cells with anti-ATP synthase F1 subunit gamma antibody (Cat#5399, 1:1,000). Nuclei were stained blue with DAPI; ATP synthase F1 subunit gamma was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-ATP synthase F1 subunit gamma antibody (Cat#5399). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP synthase F1 subunit gamma antibody (Cat#5399, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

ATP5F1C
货号：

5399
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-ATP5C1 Mouse Monoclonal Antibody
Aliases	ATP5F1C; ATP Synthase F1 Subunit Gamma; ATP5CL1; ATP5C1; ATP5C; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Gamma Polypeptide 1; ATP Synthase Subunit Gamma, Mitochondrial; F-ATPase Gamma Subunit; Mitochondrial ATP Synthase, Gamma Subunit 1; ATP Synthase Gamma Chain, Mitochondrial
Background	Gene Name: ATP5F1C NCBI Gene Entry: 509 UniProt Entry: P36542
Application Information	Molecular Weight: Predicted, 33 kDa; observed, 33 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB1325 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human ATP5C1
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:1,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of ATP synthase F1 subunit gamma expression in HepG2 cells using anti-ATP synthase F1 subunit gamma antibody (Cat#5399, 1:1,000). Green, isotype control; red, ATP synthase F1 subunit gamma.
Immunocytochemical staining of HepG2 cells with anti-ATP synthase F1 subunit gamma antibody (Cat#5399, 1:1,000). Nuclei were stained blue with DAPI; ATP synthase F1 subunit gamma was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-ATP synthase F1 subunit gamma antibody (Cat#5399). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP synthase F1 subunit gamma antibody (Cat#5399, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

ATP5F1C
货号：

5399
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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