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Anti-NDUFAF1 Rabbit Monoclonal Antibody #5478

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Flow cytometric analysis of NDUFAF1 expression in HepG2 cells using anti-NDUFAF1 antibody (Cat#5478, 1:2,000). Green, isotype control; red, NDUFAF1.
Western blotting analysis using anti-NDUFAF1 antibody (Cat#5478). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFAF1 antibody (Cat#5478, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    NDUFAF1
  • 货号:
    5478
  • 应用:
    WB, FCM
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-NDUFAF1 Rabbit Monoclonal Antibody
Aliases NDUFAF1; NADH:Ubiquinone Oxidoreductase Complex Assembly Factor 1; CIA30; CGI-65; NADH Dehydrogenase (Ubiquinone) Complex I, Assembly Factor 1; Complex I Intermediate-Associated Protein 30, Mitochondrial; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, Assembly Factor 1; NADH-Ubiquinone Oxidoreductase 1 Alpha Subcomplex, Assembly Factor 1; NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Assembly Factor 1; MC1DN11; CGI65
Background

Gene Name: NDUFAF1

NCBI Gene Entry: 51103

UniProt Entry: Q9Y375

Application Information

Molecular Weight: Predicted, 35 kDa; observed, 33 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB1675

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human NDUFAF1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of NDUFAF1 expression in HepG2 cells using anti-NDUFAF1 antibody (Cat#5478, 1:2,000). Green, isotype control; red, NDUFAF1.
  • Western blotting analysis using anti-NDUFAF1 antibody (Cat#5478). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFAF1 antibody (Cat#5478, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of NDUFAF1 expression in HepG2 cells using anti-NDUFAF1 antibody (Cat#5478, 1:2,000). Green, isotype control; red, NDUFAF1.
Western blotting analysis using anti-NDUFAF1 antibody (Cat#5478). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFAF1 antibody (Cat#5478, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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