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Anti-Lysine demethylase 4A Rabbit Monoclonal Antibody #2232

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Flow cytometric analysis of Lysine demethylase 4A expression in C2C12 cells using anti-Lysine demethylase 4A antibody (Cat#2232, 1:2,000). Green, isotype control; red, Lysine demethylase 4A.
Immunocytochemical staining of C2C12 cells with anti-Lysine demethylase 4A antibody (Cat#2232, 1:1,000) . Nuclei were stained blue with DAPI; Lysine demethylase 4A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Lysine demethylase 4A antibody (Cat#2232). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Lysine demethylase 4A antibody (Cat#2232, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    KDM4A
  • 货号:
    2232
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Lysine demethylase 4A Rabbit Monoclonal Antibody
Aliases KDM4A; Lysine Demethylase 4A; JHDM3A; KIAA0677; TDRD14A; JMJD2A; JMJD2; JmjC Domain-Containing Histone Demethylation Protein 3A; Jumonji C Domain-Containing Histone Demethylase 3A; [Histone H3]-Trimethyl-L-Lysine(36) Demethylase 4A; [Histone H3]-Trimethyl-L-Lysine(9) Demethylase 4A; Jumonji Domain-Containing Protein 2A; Lysine (K)-Specific Demethylase 4A; Lysine-Specific Demethylase 4A; Jumonji Domain Containing 2A; Tudor Domain Containing 14A; Jumonji Domain Containing 2; EC 1.14.11.66; EC 1.14.11.69; EC 1.14.11
Background

Gene Name: KDM4A

NCBI Gene Entry: 9682

UniProt Entry: O75164

Application Information

Molecular Weight: Predicted, 121 kDa; observed, 150 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB6615

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human KDM4A / JHDM3A / JMJD2A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Lysine demethylase 4A expression in C2C12 cells using anti-Lysine demethylase 4A antibody (Cat#2232, 1:2,000). Green, isotype control; red, Lysine demethylase 4A.
  • Immunocytochemical staining of C2C12 cells with anti-Lysine demethylase 4A antibody (Cat#2232, 1:1,000) . Nuclei were stained blue with DAPI; Lysine demethylase 4A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Lysine demethylase 4A antibody (Cat#2232). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Lysine demethylase 4A antibody (Cat#2232, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of Lysine demethylase 4A expression in C2C12 cells using anti-Lysine demethylase 4A antibody (Cat#2232, 1:2,000). Green, isotype control; red, Lysine demethylase 4A.
Immunocytochemical staining of C2C12 cells with anti-Lysine demethylase 4A antibody (Cat#2232, 1:1,000) . Nuclei were stained blue with DAPI; Lysine demethylase 4A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Lysine demethylase 4A antibody (Cat#2232). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Lysine demethylase 4A antibody (Cat#2232, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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