Flow cytometric analysis of HLF expression in HepG2 cells using anti-HLF antibody (Cat#5490, 1:2,000). Green, isotype control; red, HLF.
Immunocytochemical staining of HepG2 cells with anti-HLF antibody (Cat#5490, 1:1,000) . Nuclei were stained blue with DAPI; HLF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-HLF antibody (Cat#5490). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HLF antibody (Cat#5490, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
HLF; HLF Transcription Factor, PAR BZIP Family Member; Hepatic Leukemia Factor; HLF, PAR BZIP Transcription Factor; PAR BZIP Transcription Factor; MGC33822; E2A-HLF Fusion Transcript Protein
Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human Hepatic leukemia factor
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000
Note
This product is for research use only.
Picture
Flow cytometric analysis of HLF expression in HepG2 cells using anti-HLF antibody (Cat#5490, 1:2,000). Green, isotype control; red, HLF.
Immunocytochemical staining of HepG2 cells with anti-HLF antibody (Cat#5490, 1:1,000) . Nuclei were stained blue with DAPI; HLF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-HLF antibody (Cat#5490). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HLF antibody (Cat#5490, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of HLF expression in HepG2 cells using anti-HLF antibody (Cat#5490, 1:2,000). Green, isotype control; red, HLF.
Immunocytochemical staining of HepG2 cells with anti-HLF antibody (Cat#5490, 1:1,000) . Nuclei were stained blue with DAPI; HLF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-HLF antibody (Cat#5490). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HLF antibody (Cat#5490, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
