Flow cytometric analysis of Replication protein A2 expression in HepG2 cells using anti-Replication protein A2 antibody (Cat#5501, 1:2,000). Green, isotype control; red, Replication protein A2.
Immunocytochemical staining of HepG2 cells with anti-Replication protein A2 antibody (Cat#5501,1:1,000) . Nuclei were stained blue with DAPI; Replication protein A2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-replication protein A2 antibody (Cat#5501). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-replication protein A2 antibody (Cat#5501, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
RPA2; Replication Protein A2; Replication Protein A 32 KDa Subunit; Replication Protein A 34 KDa Subunit; Replication Factor A Protein 2; RF-A Protein 2; RP-A P32; RP-A P34; REPA2; RPA32; Replication Protein A2 (32kD); Replication Protein A2, 32kDa; RPA34
Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human RPA2
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000
Note
This product is for research use only.
Picture
Flow cytometric analysis of Replication protein A2 expression in HepG2 cells using anti-Replication protein A2 antibody (Cat#5501, 1:2,000). Green, isotype control; red, Replication protein A2.
Immunocytochemical staining of HepG2 cells with anti-Replication protein A2 antibody (Cat#5501,1:1,000) . Nuclei were stained blue with DAPI; Replication protein A2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-replication protein A2 antibody (Cat#5501). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-replication protein A2 antibody (Cat#5501, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Replication protein A2 expression in HepG2 cells using anti-Replication protein A2 antibody (Cat#5501, 1:2,000). Green, isotype control; red, Replication protein A2.
Immunocytochemical staining of HepG2 cells with anti-Replication protein A2 antibody (Cat#5501,1:1,000) . Nuclei were stained blue with DAPI; Replication protein A2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-replication protein A2 antibody (Cat#5501). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-replication protein A2 antibody (Cat#5501, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
