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Anti-Glucocorticoid Receptor Rabbit Monoclonal Antibody #5631

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Flow cytometric analysis of Glucocorticoid Receptor expression in HT-1080 cells using Glucocorticoid Receptor antibody (Cat#5631,1:2,000). Green, isotype control; red, Glucocorticoid Receptor.

Immunocytochemical staining of HT-1080 cells with Glucocorticoid Receptor antibody (Cat#5631, 1:1,000). Nuclei were stained blue with DAPI; Glucocorticoid Receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Glucocorticoid Receptor antibody (Cat#5631). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Glucocorticoid Receptor antibody (Cat#5631,1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#MR226).

基因名：

NR3C1
货号：

5631
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-Glucocorticoid Receptor Rabbit Monoclonal Antibody
Aliases	Nuclear Receptor Subfamily 3 Group C Member 1; GR; Glucocorticoid Receptor; GRL; Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor); Nuclear Receptor Subfamily 3 Group C Member 1 Variant HGR-B(54); Nuclear Receptor Subfamily 3 Group C Member 1 Variant HGR-B(77); Nuclear Receptor Subfamily 3 Group C Member 1 Variant HGR-B(93); Nuclear Receptor Subfamily 3, Group C, Member 1; GCRST; GCCR; GCR
Background	Gene Name: NR3C1 NCBI Gene Entry: 2908 UniProt Entry: P04150
Application Information	Molecular Weight: Predicted, 86 kDa; observed, 86 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB935 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human GR
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:2,000-1:10,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Glucocorticoid Receptor expression in HT-1080 cells using Glucocorticoid Receptor antibody (Cat#5631,1:2,000). Green, isotype control; red, Glucocorticoid Receptor.
Immunocytochemical staining of HT-1080 cells with Glucocorticoid Receptor antibody (Cat#5631, 1:1,000). Nuclei were stained blue with DAPI; Glucocorticoid Receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Glucocorticoid Receptor antibody (Cat#5631). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Glucocorticoid Receptor antibody (Cat#5631,1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#MR226).

Citation(0)

基因名：

NR3C1
货号：

5631
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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