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Anti-FIG4 Rabbit Monoclonal Antibody #5634

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Western blotting analysis using anti-FIG4 antibody (Cat#5634). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FIG4 antibody (Cat#5634, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with anti-FIG4 antibody (Cat#5634, 1:1,000) . Nuclei were stained blue with DAPI; FIG4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-FIG4 antibody (Cat#5634). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FIG4 antibody (Cat#5634, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of FIG4 expression in C2C12 cells using anti-FIG4 antibody (Cat#5634, 1:2,000). Green, isotype control; red, FIG4.
  • 基因名：
    FIG4
  • 货号：
    5634
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-FIG4 Rabbit Monoclonal Antibody
Aliases FIG4; FIG4 Phosphoinositide 5-Phosphatase; KIAA0274; SAC3; DJ249I4.1; ALS11; CMT4J; Phosphatidylinositol 3,5-Bisphosphate 5-Phosphatase; Polyphosphoinositide Phosphatase; SAC Domain-Containing Protein 3; Serine-Protein Phosphatase FIG4; EC 3.1.3.36; HSac3; FIG4 Homolog, SAC1 Lipid Phosphatase Domain Containing (S. Cerevisiae); FIG4 Homolog, SAC1 Domain Containing Lipid Phosphatase; FIG4 Homolog, SAC1 Lipid Phosphatase Domain Containing; FIG4 Homolog, SAC Domain Containing Lipid Phosphatase; Sac Domain-Containing Inositol Phosphatase 3; FIG4 Homolog (S. Cerevisiae); EC 3.1.3.86; EC 3.1.3.16; EC 3.1.3.-; EC 3.1.3; BTOP; BOP; YVS
Background

Gene Name: FIG4

NCBI Gene Entry: 9896

UniProt Entry: Q92562
Application Information

Molecular Weight: Predicted, 104 kDa; observed, 100 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4620

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human FIG4
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-FIG4 antibody (Cat#5634). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FIG4 antibody (Cat#5634, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with anti-FIG4 antibody (Cat#5634, 1:1,000) . Nuclei were stained blue with DAPI; FIG4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-FIG4 antibody (Cat#5634). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FIG4 antibody (Cat#5634, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of FIG4 expression in C2C12 cells using anti-FIG4 antibody (Cat#5634, 1:2,000). Green, isotype control; red, FIG4.
Citation(0)
  • 基因名：
    FIG4
  • 货号：
    5634
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Western blotting analysis using anti-FIG4 antibody (Cat#5634). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FIG4 antibody (Cat#5634, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with anti-FIG4 antibody (Cat#5634, 1:1,000) . Nuclei were stained blue with DAPI; FIG4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-FIG4 antibody (Cat#5634). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FIG4 antibody (Cat#5634, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of FIG4 expression in C2C12 cells using anti-FIG4 antibody (Cat#5634, 1:2,000). Green, isotype control; red, FIG4.
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