Flow cytometric analysis of ATG7 expression in HT-1080 cells using ATG7 antibody (Cat#61177, 1:2,000). Green, isotype control; red, ATG7.
Immunocytochemical staining of HT-1080 cells with ATG7 antibody (Cat#61177, 1:1,000). Nuclei were stained blue with DAPI; ATG7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-ATG7 antibody (Cat#61177). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATG7 antibody (Cat#61177, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-ATG7 antibody (Cat#61177). ATG7 expression in wild type (WT) and ATG7 shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-ATG7 antibody (Cat#61177, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of ATG7 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) 293T cells were stained with anti-ATG7 antibody (Cat#61177, 1:2,000) and analyzed using BD flow cytometer.
