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KD-Validated Anti-c-Kit Rabbit Monoclonal Antibody #61209

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Western blotting analysis using anti-c-Kit antibody (Cat#61209). C-Kit expression in wild type (WT) and KIT shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-c-Kit antibody (Cat#61209, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-c-kit antibody (Cat#61209). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-c-kit antibody (Cat#61209, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    KIT
  • 货号:
    61209
  • 应用:
    WB
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-c-Kit Rabbit Monoclonal Antibody
Aliases KIT; KIT Proto-Oncogene, Receptor Tyrosine Kinase; SCFR; V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog; Mast/Stem Cell Growth Factor Receptor Kit ; CD117 ; PBT; Tyrosine-Protein Kinase Kit; Piebald Trait Protein; Proto-Oncogene C-Kit; EC 2.7.10.1; P145 C-Kit; C-Kit; V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene-Like Protein ; Proto-Oncogene Tyrosine-Protein Kinase Kit; C-Kit Protooncogene; Piebald Trait; CD117 Antigen ; EC 2.7.10; MASTC; C-KIT
Background

Gene Name: KIT

NCBI Gene Entry: 3815

UniProt Entry: P10721

Application Information

Molecular Weight: Predicted, 110 kDa; observed, 120-145 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB16200

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human c-Kit
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-c-Kit antibody (Cat#61209). C-Kit expression in wild type (WT) and KIT shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-c-Kit antibody (Cat#61209, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-c-kit antibody (Cat#61209). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-c-kit antibody (Cat#61209, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Western blotting analysis using anti-c-Kit antibody (Cat#61209). C-Kit expression in wild type (WT) and KIT shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-c-Kit antibody (Cat#61209, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-c-kit antibody (Cat#61209). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-c-kit antibody (Cat#61209, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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