KD-Validated Anti-ARAF Rabbit Monoclonal Antibody - 产品中心

KD-Validated Anti-ARAF Rabbit Monoclonal Antibody #61218

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Flow cytometric analysis of ARAF expression in HepG2 cells using anti-ARAF antibody (Cat# 61218, 1:2,000). Green, isotype control; red, ARAF.

Western blotting analysis using anti-ARAF antibody (Cat#61218). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ARAF antibody (Cat#61218, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-ARAF antibody (Cat#61218). ARAF expression in wild type (WT) and ARAF knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ARAF antibody (Cat#61218, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

ARAF
货号：

61218
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-ARAF Rabbit Monoclonal Antibody
Aliases	ARAF; A-Raf Proto-Oncogene, Serine/Threonine Kinase; ARAF1; V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog 1; Serine/Threonine-Protein Kinase A-Raf; Proto-Oncogene A-Raf-1; Proto-Oncogene Pks; EC 2.7.11.1; A-Raf; PKS2; V-Raf Murine Sarcoma 3611 Viral Oncogene-Like Protein; A-Raf Proto-Oncogene Serine/Threonine-Protein Kinase; Ras-Binding Protein DA-Raf; Proto-Oncogene A-Raf; Oncogene ARAF1; EC 2.7.11; RAFA1; PKS
Background	Gene Name: ARAF NCBI Gene Entry: 369 UniProt Entry: P10398
Application Information	Molecular Weight: Predicted, 68 kDa, observed, 68 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB440 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human A-RAF
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of ARAF expression in HepG2 cells using anti-ARAF antibody (Cat# 61218, 1:2,000). Green, isotype control; red, ARAF.
Western blotting analysis using anti-ARAF antibody (Cat#61218). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ARAF antibody (Cat#61218, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-ARAF antibody (Cat#61218). ARAF expression in wild type (WT) and ARAF knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ARAF antibody (Cat#61218, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

ARAF
货号：

61218
应用：

WB, FCM
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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