KD-Validated Anti-BAX Rabbit Monoclonal Antibody - 产品中心

KD-Validated Anti-BAX Rabbit Monoclonal Antibody #61220

一键复制产品信息

返回列表

Flow cytometric analysis of BAX expression in HT-1080 cells using anti-BAX antibody (Cat# 61220, 1:2,000). Green, isotype control; red, BAX.

Immunocytochemical staining of HT-1080 cells with anti-BAX antibody (Cat#61220, 1:1,000). Nuclei were stained blue with DAPI; BAX was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-BAX antibody (Cat#61220). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BAX antibody (Cat#61220, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-BAX antibody (Cat#61220). BAX expression in wild type (WT) and BAX knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-BAX antibody (Cat#61220, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

BAX
货号：

61220
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-BAX Rabbit Monoclonal Antibody
Aliases	BAX; BCL2 Associated X, Apoptosis Regulator; BCL2L4; BCL2 Associated X Protein; Apoptosis Regulator BAX; Bcl-2-Like Protein 4; Bcl2-L-4; BCL2-Associated X Protein Omega; BCL2-Associated X Protein; Baxdelta2G9omega; Baxdelta2omega; Baxdelta2G9
Background	Gene Name: BAX NCBI Gene Entry: 581 UniProt Entry: Q07812
Application Information	Molecular Weight: Predicted, 21 kDa, observed, 21 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB450 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Bax
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of BAX expression in HT-1080 cells using anti-BAX antibody (Cat# 61220, 1:2,000). Green, isotype control; red, BAX.
Immunocytochemical staining of HT-1080 cells with anti-BAX antibody (Cat#61220, 1:1,000). Nuclei were stained blue with DAPI; BAX was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-BAX antibody (Cat#61220). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BAX antibody (Cat#61220, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-BAX antibody (Cat#61220). BAX expression in wild type (WT) and BAX knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-BAX antibody (Cat#61220, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

BAX
货号：

61220
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

首页

关于我们

善本标准

产品中心

定制服务

信号通路

促销活动

技术支持

新闻资讯

首页