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KD-Validated Anti-HIF1AN Rabbit Monoclonal Antibody #62181

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Flow cytometric analysis of HIF1AN expression in HeLa cells using HIF1AN antibody (Cat#62181, 1:2,000). GrHIF1ANotype control; red, HIF1AN.
Immunocytochemical staining of HeLa cells with anti-HIF1AN antibody (Cat#62181, 1:1,000). Nuclei were stained blue with DAPI; HIF1AN was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-HIF1AN antibody (Cat#62181). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HIF1AN antibody (Cat#62181, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-HIF1AN antibody (Cat#62181). HIF1AN expression in wild type (WT) and HIF1AN shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HIF1AN antibody (Cat#62181, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    HIF1AN
  • 货号：
    62181
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-HIF1AN Rabbit Monoclonal Antibody
Aliases HIF1AN; Hypoxia Inducible Factor 1 Subunit Alpha Inhibitor; FIH1; Hypoxia-Inducible Factor Asparagine Hydroxylase; Hypoxia-Inducible Factor 1-Alpha Inhibitor; Peptide-Aspartate Beta-Dioxygenase; Factor Inhibiting HIF-1; FLJ20615; FLJ22027; FIH-1; Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor; Factor Inhibiting HIF1; DKFZp762F1811; DKFZP762F1811; EC 1.14.11.30; EC 1.14.11.N4
Background

Gene Name: HIF1AN

NCBI Gene Entry: 55662

UniProt Entry: Q9NWT6
Application Information

Molecular Weight: Predicted, 40 kDa, observed, 40 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB6385

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human HIF1AN
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of HIF1AN expression in HeLa cells using HIF1AN antibody (Cat#62181, 1:2,000). GrHIF1ANotype control; red, HIF1AN.
  • Immunocytochemical staining of HeLa cells with anti-HIF1AN antibody (Cat#62181, 1:1,000). Nuclei were stained blue with DAPI; HIF1AN was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-HIF1AN antibody (Cat#62181). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HIF1AN antibody (Cat#62181, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-HIF1AN antibody (Cat#62181). HIF1AN expression in wild type (WT) and HIF1AN shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HIF1AN antibody (Cat#62181, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    HIF1AN
  • 货号：
    62181
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of HIF1AN expression in HeLa cells using HIF1AN antibody (Cat#62181, 1:2,000). GrHIF1ANotype control; red, HIF1AN.
Immunocytochemical staining of HeLa cells with anti-HIF1AN antibody (Cat#62181, 1:1,000). Nuclei were stained blue with DAPI; HIF1AN was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-HIF1AN antibody (Cat#62181). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HIF1AN antibody (Cat#62181, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-HIF1AN antibody (Cat#62181). HIF1AN expression in wild type (WT) and HIF1AN shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HIF1AN antibody (Cat#62181, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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