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KD-Validated Anti-Membrane Metalloendopeptidase Rabbit Monoclonal Antibody #61234

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Western blotting analysis using anti-CD10 antibody (Cat#61234). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD10 antibody (Cat#61234, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-CD10 antibody (Cat#61234). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD10 antibody (Cat#61234, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-CD10 antibody (Cat#61234). CD10 expression in wild type (WT) and CD10 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CD10 antibody (Cat#61234, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    MME
  • 货号：
    61234
  • 应用：
    WB
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Membrane Metalloendopeptidase Rabbit Monoclonal Antibody
Aliases Membrane Metalloendopeptidase; CALLA; NEP; Neprilysin; CD10; Common Acute Lymphocytic Leukemia Antigen; Neutral Endopeptidase 24.11; Skin Fibroblast Elastase; Neutral Endopeptidase; Atriopeptidase; Enkephalinase; EC 3.4.24.11; SFE; Membrane Metallo-Endopeptidase (Neutral Endopeptidase, Enkephalinase, CALLA, CD10); Neprilysin-390; Neprilysin-411; CD10 Antigen; EC 3.4.24; CMT2T; SCA43; EPN
Background

Gene Name: MME

NCBI Gene Entry: 4311

UniProt Entry: P08473
Application Information

Molecular Weight: Predicted, 86 kDa; observed, 100 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB480

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human CD10
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-CD10 antibody (Cat#61234). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD10 antibody (Cat#61234, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-CD10 antibody (Cat#61234). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD10 antibody (Cat#61234, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-CD10 antibody (Cat#61234). CD10 expression in wild type (WT) and CD10 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CD10 antibody (Cat#61234, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    MME
  • 货号：
    61234
  • 应用：
    WB
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Western blotting analysis using anti-CD10 antibody (Cat#61234). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD10 antibody (Cat#61234, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-CD10 antibody (Cat#61234). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD10 antibody (Cat#61234, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-CD10 antibody (Cat#61234). CD10 expression in wild type (WT) and CD10 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CD10 antibody (Cat#61234, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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