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Anti-Phospho-GAP43 (S41) Rabbit Polyclonal Antibody #U0751

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Immunocytochemical analysis of GAP43 (Phospho-S41) staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of GAP43 (Phospho-S41) expression in HeLa PMA-treated (A), SHSY5Y (B) whole cell lysates.
  • 基因名：
    GAP43
  • 货号：
    U0751
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R, B, C, Mk, Z
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00

Information

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Information
Product Name Anti-Phospho-GAP43 (S41) Rabbit Polyclonal Antibody
Aliases Neuromodulin; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46
Background

Gene Name: GAP43

NCBI Gene Entry: 2596

UniProt Entry: P17677
Application Information

Molecular Weight: Predicted, 24 kDa; observed, 24 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, bovine, chicken, monkey, zebrafish

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Phospho-GAP43 (S41)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,000
Immunocytochemistry (IC): 1:50-1:100
Note This product is for research use only.
Picture
  • Immunocytochemical analysis of GAP43 (Phospho-S41) staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
  • Western blotting analysis of GAP43 (Phospho-S41) expression in HeLa PMA-treated (A), SHSY5Y (B) whole cell lysates.
Citation(0)
  • 基因名：
    GAP43
  • 货号：
    U0751
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R, B, C, Mk, Z
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00
RELATED PRODUCTS
Immunocytochemical analysis of GAP43 (Phospho-S41) staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of GAP43 (Phospho-S41) expression in HeLa PMA-treated (A), SHSY5Y (B) whole cell lysates.
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