Flow cytometric analysis of MacroH2A.1 Histone expression in HAP-1 cells usiMacroH2A.1 Histone0 α antibody (Cat# 61249, 1:2,000). Green, isotype control; red, MacroH2A.1 Histone.
Western blotting analysis using anti-MacroH2A.1 Histone antibody (Cat#61249). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MacroH2A.1 Histone antibody (Cat#61249, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-macroH2A.1 histone antibody (Cat#61249). MacroH2A.1 histone expression in wild type (WT) and macroH2A.1 histone (H2AFY) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-macroH2A.1 histone antibody (Cat#61249, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of MacroH2A.1 Histone knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-MacroH2A.1 Histone antibody (Cat#61249, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-MacroH2A.1 Histone antibody (Cat#61249, 1:1,000), Top panel: wild-type (WT); Bottom panal: MacroH2A.1 Histone shRNA knockdown (KD). Nuclei were stained blue with DAPI; MacroH2A.1 Histone was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
