Flow cytometric analysis of PACSIN3 expression in HepG2 cells using anti-PACSIN3 antibody (Cat# 61258, 1:2,000). Green, isotype control; red, PACSIN3.
Western blotting analysis using anti-PACSIN3 antibody (Cat#61258). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PACSIN3 antibody (Cat#61258, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-PACSIN3 antibody (Cat#61258). PACSIN3 expression in wild type (WT) and PACSIN3 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PACSIN3 antibody (Cat#61258, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
PACSIN3; Protein Kinase C And Casein Kinase SubstRate In Neurons 3; SDPIII; Protein Kinase C And Casein Kinase SubstRate In Neurons Protein 3; SH3 Domain-Containing Protein 6511; Syndapin III
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human PACSIN3
Isotype
Mouse IgG2a
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:2,500 Flow Cytometry (FCM): 1:2,000
Note
This product is for research use only.
Picture
Flow cytometric analysis of PACSIN3 expression in HepG2 cells using anti-PACSIN3 antibody (Cat# 61258, 1:2,000). Green, isotype control; red, PACSIN3.
Western blotting analysis using anti-PACSIN3 antibody (Cat#61258). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PACSIN3 antibody (Cat#61258, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-PACSIN3 antibody (Cat#61258). PACSIN3 expression in wild type (WT) and PACSIN3 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PACSIN3 antibody (Cat#61258, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PACSIN3 expression in HepG2 cells using anti-PACSIN3 antibody (Cat# 61258, 1:2,000). Green, isotype control; red, PACSIN3.
Western blotting analysis using anti-PACSIN3 antibody (Cat#61258). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PACSIN3 antibody (Cat#61258, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-PACSIN3 antibody (Cat#61258). PACSIN3 expression in wild type (WT) and PACSIN3 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PACSIN3 antibody (Cat#61258, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
