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KD-Validated Anti-PUS7 Mouse Monoclonal Antibody #61271

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Western blotting analysis using anti-pseudouridine synthase 7 antibody (Cat#61271). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-pseudouridine synthase 7 antibody (Cat#61271, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-pseudouridine synthase 7 antibody (Cat#61271). Pseudouridine synthase 7 expression in wild-type (WT) and pseudouridine synthase 7 (PUS7) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-pseudouridine synthase 7 antibody (Cat#61271, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Pseudouridine synthase 7 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Pseudouridine synthase 7 antibody (Cat#61271, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Pseudouridine synthase 7 antibody (Cat#61271, 1:1,000), Top panel: wild-type (WT); Bottom panal: Pseudouridine synthase 7 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Pseudouridine synthase 7 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • 基因名:
    PUS7
  • 货号:
    61271
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-PUS7 Mouse Monoclonal Antibody
Aliases PUS7; Pseudouridine Synthase 7; Pseudouridylate Synthase 7 (Putative); Pseudouridylate Synthase 7 Homolog; FLJ20485; Pseudouridylate Synthase 7 Homolog (S. Cerevisiae); Pseudouridylate Synthase 7; EC 5.4.99.-; KIAA1897; IDDABS
Background

Gene Name: PUS7

NCBI Gene Entry: 54517

UniProt Entry: Q96PZ0

Application Information

Molecular Weight: Predicted, 75 kDa; observed, 75 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB4125

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen Recombinant protein of human PUS7
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-pseudouridine synthase 7 antibody (Cat#61271). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-pseudouridine synthase 7 antibody (Cat#61271, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-pseudouridine synthase 7 antibody (Cat#61271). Pseudouridine synthase 7 expression in wild-type (WT) and pseudouridine synthase 7 (PUS7) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-pseudouridine synthase 7 antibody (Cat#61271, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of Pseudouridine synthase 7 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Pseudouridine synthase 7 antibody (Cat#61271, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HeLa cells using anti-Pseudouridine synthase 7 antibody (Cat#61271, 1:1,000), Top panel: wild-type (WT); Bottom panal: Pseudouridine synthase 7 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Pseudouridine synthase 7 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Citation(0)
Western blotting analysis using anti-pseudouridine synthase 7 antibody (Cat#61271). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-pseudouridine synthase 7 antibody (Cat#61271, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-pseudouridine synthase 7 antibody (Cat#61271). Pseudouridine synthase 7 expression in wild-type (WT) and pseudouridine synthase 7 (PUS7) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-pseudouridine synthase 7 antibody (Cat#61271, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Pseudouridine synthase 7 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Pseudouridine synthase 7 antibody (Cat#61271, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Pseudouridine synthase 7 antibody (Cat#61271, 1:1,000), Top panel: wild-type (WT); Bottom panal: Pseudouridine synthase 7 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Pseudouridine synthase 7 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
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