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KD-Validated Anti-MAP2K1 Rabbit Monoclonal Antibody #61295

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Flow cytometric analysis of MAP2K1 expression in C2C12 cells using MAP2K1 antibody (Cat#61295, 1:2,000). Green, isotype control; red, MAP2K1.
Immunocytochemical staining of C2C12 cells with MAP2K1 antibody (Cat#61295, 1:1,000). Nuclei were stained blue with DAPI; MAP2K1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-MAP2K1 antibody (Cat#61295). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAP2K1 antibody (Cat#61295, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-MAP2K1 antibody (Cat#61295). MAP2K1 expression in wild type (WT) and MAP2K1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MAP2K1 antibody (Cat#61295, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of MAP2K1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-MAP2K1 antibody (Cat#61295, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-MAP2K1 antibody (Cat#61295, 1:1,000), Top panel: wild-type (WT); Bottom panal:MAP2K1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;MAP2K1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • 基因名：
    MAP2K1
  • 货号：
    61295
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-MAP2K1 Rabbit Monoclonal Antibody
Aliases MAP2K1; Mitogen-Activated Protein Kinase Kinase 1; MEK1; Dual Specificity Mitogen-Activated Protein Kinase Kinase 1; MAPK/ERK Kinase 1; MAPKK1; PRKMK1; MKK1; ERK Activator Kinase 1; MAP Kinase Kinase 1; EC 2.7.12.2; MAPKK 1; MEK 1; Protein Kinase, Mitogen-Activated, Kinase 1 (MAP Kinase Kinase 1); CFC3; MEL
Background

Gene Name: MAP2K1

NCBI Gene Entry: 5604

UniProt Entry: Q02750
Application Information

Molecular Weight: Predicted, 43 kDa, observed, 45 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2475

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human MEK1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of MAP2K1 expression in C2C12 cells using MAP2K1 antibody (Cat#61295, 1:2,000). Green, isotype control; red, MAP2K1.
  • Immunocytochemical staining of C2C12 cells with MAP2K1 antibody (Cat#61295, 1:1,000). Nuclei were stained blue with DAPI; MAP2K1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-MAP2K1 antibody (Cat#61295). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAP2K1 antibody (Cat#61295, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-MAP2K1 antibody (Cat#61295). MAP2K1 expression in wild type (WT) and MAP2K1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MAP2K1 antibody (Cat#61295, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of MAP2K1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-MAP2K1 antibody (Cat#61295, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HeLa cells using anti-MAP2K1 antibody (Cat#61295, 1:1,000), Top panel: wild-type (WT); Bottom panal:MAP2K1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;MAP2K1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Citation(0)
  • 基因名：
    MAP2K1
  • 货号：
    61295
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of MAP2K1 expression in C2C12 cells using MAP2K1 antibody (Cat#61295, 1:2,000). Green, isotype control; red, MAP2K1.
Immunocytochemical staining of C2C12 cells with MAP2K1 antibody (Cat#61295, 1:1,000). Nuclei were stained blue with DAPI; MAP2K1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-MAP2K1 antibody (Cat#61295). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAP2K1 antibody (Cat#61295, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-MAP2K1 antibody (Cat#61295). MAP2K1 expression in wild type (WT) and MAP2K1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MAP2K1 antibody (Cat#61295, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of MAP2K1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-MAP2K1 antibody (Cat#61295, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-MAP2K1 antibody (Cat#61295, 1:1,000), Top panel: wild-type (WT); Bottom panal:MAP2K1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;MAP2K1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
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