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KD-Validated Anti-CDC34 Rabbit Monoclonal Antibody #61321

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Flow cytometric analysis of CDC34 expression in HT-1080 cells using CDC34 antibody (Cat#61321, 1:2,000). Green, isotype control; red, CDC34.
Immunocytochemical staining of HT-1080 cells with anti-CDC34 antibody (Cat#61321, 1:1,000). Nuclei were stained blue with DAPI; CDC34 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-CDC34 antibody (Cat#61321). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC34 antibody (Cat#61321, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-CDC34 antibody (Cat#61321). CDC34 expression in wild-type (WT) and CDC34 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CDC34 antibody (Cat#61321, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    CDC34
  • 货号：
    61321
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-CDC34 Rabbit Monoclonal Antibody
Aliases CDC34; Cell Division Cycle 34, Ubiqiutin Conjugating Enzyme; UBE2R1; E2-CDC34; UBC3; (E3-Independent) E2 Ubiquitin-Conjugating Enzyme R1; Ubiquitin-Conjugating Enzyme E2-CDC34; Ubiquitin-Conjugating Enzyme E2 R1; E2 Ubiquitin-Conjugating Enzyme R1; Ubiquitin-Protein Ligase R1; UBCH3; Ubiquitin-Conjugating Enzyme E2-32 KDA Complementing; Ubiquitin-Conjugating Enzyme E2-32 KDa Complementing; Ubiquitin Conjugating Enzyme E2 R1; Cell Division Cycle 34 Homolog; Ubiquitin Carrier Protein; EC 2.3.2.23; EC 2.3.2.24; EC 6.3.2.19
Background

Gene Name: CDC34

NCBI Gene Entry: 997

UniProt Entry: P49427
Application Information

Molecular Weight: Predicted, 27 kDa; observed, 34 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2245

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human CDC34
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of CDC34 expression in HT-1080 cells using CDC34 antibody (Cat#61321, 1:2,000). Green, isotype control; red, CDC34.
  • Immunocytochemical staining of HT-1080 cells with anti-CDC34 antibody (Cat#61321, 1:1,000). Nuclei were stained blue with DAPI; CDC34 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-CDC34 antibody (Cat#61321). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC34 antibody (Cat#61321, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-CDC34 antibody (Cat#61321). CDC34 expression in wild-type (WT) and CDC34 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CDC34 antibody (Cat#61321, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    CDC34
  • 货号：
    61321
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of CDC34 expression in HT-1080 cells using CDC34 antibody (Cat#61321, 1:2,000). Green, isotype control; red, CDC34.
Immunocytochemical staining of HT-1080 cells with anti-CDC34 antibody (Cat#61321, 1:1,000). Nuclei were stained blue with DAPI; CDC34 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-CDC34 antibody (Cat#61321). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC34 antibody (Cat#61321, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-CDC34 antibody (Cat#61321). CDC34 expression in wild-type (WT) and CDC34 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CDC34 antibody (Cat#61321, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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