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KD-Validated Anti-CC2D1A Rabbit Monoclonal Antibody #61327

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Flow cytometric analysis of CC2D1A expression in C2C12 cells using CC2D1A antibody (Cat#61327, 1:2,000). Green, isotype control; red, CC2D1A.
Immunocytochemical staining of HepG2 cells with anti-CC2D1A antibody (Cat#61327, 1:1000) . Nuclei were stained blue with DAPI; CC2D1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-CC2D1A antibody (Cat#61327). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CC2D1A antibody (Cat#61327, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). CC2D1A, coiled-coil and C2 domain containing 1A.
Western blotting analysis using anti-CC2D1A antibody (Cat#61327). CC2D1A expression in wild type (WT) and CC2D1A shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CC2D1A antibody (Cat#61327, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    CC2D1A
  • 货号：
    61327
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-CC2D1A Rabbit Monoclonal Antibody
Aliases CC2D1A; Coiled-Coil And C2 Domain Containing 1A; Five Prime Repressor Element Under Dual; Repression-Binding Protein 1; Akt Kinase-Interacting Protein 1; MRT3; TAPE; Coiled-Coil And C2 Domain-Containing Protein 1A; FRE Under Dual Repression-Binding Protein 1; Putative NF-Kappa-B-Activating Protein 023N; TBK1-Associated Protein In Endolysosomes 2; Lethal (2) Giant Discs Homolog 2; FLJ20241; Freud-1; FREUD-1; Aki-1; Lgd2; Five Repressor Element Under Dual Repression-Binding Protein 1; Mental Retardation, Nonsyndromic, Autosomal Recessive, 3; Putative NFkB Activating Protein; Freud-1/Aki1; AKI-1; LGD2; AKI1
Background

Gene Name: CC2D1A

NCBI Gene Entry: 54862

UniProt Entry: Q6P1N0
Application Information

Molecular Weight: Predicted, 104 kDa, observed, 130 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3045

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from CC2D1A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of CC2D1A expression in C2C12 cells using CC2D1A antibody (Cat#61327, 1:2,000). Green, isotype control; red, CC2D1A.
  • Immunocytochemical staining of HepG2 cells with anti-CC2D1A antibody (Cat#61327, 1:1000) . Nuclei were stained blue with DAPI; CC2D1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-CC2D1A antibody (Cat#61327). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CC2D1A antibody (Cat#61327, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). CC2D1A, coiled-coil and C2 domain containing 1A.
  • Western blotting analysis using anti-CC2D1A antibody (Cat#61327). CC2D1A expression in wild type (WT) and CC2D1A shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CC2D1A antibody (Cat#61327, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    CC2D1A
  • 货号：
    61327
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of CC2D1A expression in C2C12 cells using CC2D1A antibody (Cat#61327, 1:2,000). Green, isotype control; red, CC2D1A.
Immunocytochemical staining of HepG2 cells with anti-CC2D1A antibody (Cat#61327, 1:1000) . Nuclei were stained blue with DAPI; CC2D1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-CC2D1A antibody (Cat#61327). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CC2D1A antibody (Cat#61327, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). CC2D1A, coiled-coil and C2 domain containing 1A.
Western blotting analysis using anti-CC2D1A antibody (Cat#61327). CC2D1A expression in wild type (WT) and CC2D1A shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CC2D1A antibody (Cat#61327, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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