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KD-Validated Anti-BAG1 Rabbit Monoclonal Antibody #61340

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Flow cytometric analysis of BAG1 expression in C2C12 cells using BAG1 antibody (Cat#61340, 1:2,000). Green, isotype control; red, BAG1.

Immunocytochemical staining of C2C12 cells BAG1 antibody (Cat#61340, 1:1,000). Nuclei were stained blue with DAPI; BAG1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-BAG1 antibody (Cat#61340). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BAG1 antibody (Cat#61340, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-BAG1 antibody (Cat#61340). BAG1 expression in wild type (WT) and BAG1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-BAG1 antibody (Cat#61340, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

BAG1
货号：

61340
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

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Citation(0)

Information

Product Name	KD-Validated Anti-BAG1 Rabbit Monoclonal Antibody
Aliases	BAG1; BAG Cochaperone 1; BAG Family Molecular Chaperone Regulator 1; BCL2 Associated Athanogene 1; BAG-1; HAP; Glucocortoid Receptor-Associated Protein RAP46; Bcl-2 Associating Athanogene-1 Protein; Receptor-Associated Protein, 46-K; Bcl-2-Associated Athanogene 1; BCL2 Associated Athanogene; BCL2-Associated Athanogene; Bcl-2-Binding Protein; RAP46
Background	Gene Name: BAG1 NCBI Gene Entry: 573 UniProt Entry: Q99933
Application Information	Molecular Weight: Predicted, 39 kDa, observed, 33, 46,50 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2995 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from Bag1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of BAG1 expression in C2C12 cells using BAG1 antibody (Cat#61340, 1:2,000). Green, isotype control; red, BAG1.
Immunocytochemical staining of C2C12 cells BAG1 antibody (Cat#61340, 1:1,000). Nuclei were stained blue with DAPI; BAG1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-BAG1 antibody (Cat#61340). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BAG1 antibody (Cat#61340, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-BAG1 antibody (Cat#61340). BAG1 expression in wild type (WT) and BAG1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-BAG1 antibody (Cat#61340, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

BAG1
货号：

61340
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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