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Anti-Myosin Light Chain 12B Rabbit Monoclonal Antibody #2271

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Flow cytometric analysis of Myosin light chain 12B expression in H9c2 cells using Myosin light chain 12B antibody (Cat#2271, 1:2,000). Green, isotype control; red, Myosin light chain 12B.
Immunocytochemical staining of H9C2 cells with anti-Myosin light chain 12B antibody (Cat#2271, 1:1,000). Nuclei were stained blue with DAPI; Myosin light chain 12B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Myosin light chain 12B antibody (Cat#2271). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Myosin light chain 12B antibody (Cat#2271, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    MYL12B
  • 货号:
    2271
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Myosin Light Chain 12B Rabbit Monoclonal Antibody
Aliases Myosin Light Chain 12B; MRLC2; Myosin Regulatory Light Chain 20 KDa; Myosin, Light Chain 12B, Regulatory; Myosin Regulatory Light Chain MRLC2; Myosin Regulatory Light Chain 12B; Myosin Regulatory Light Chain 2; SHUJUN-1; MLC-2A; MLC-2; MLC20; Myosin Regulatory Light Chain 2-B, Smooth Muscle Isoform; MYLC2B; MLC-B
Background

Gene Name: MYL12B

NCBI Gene Entry: 103910

UniProt Entry: O14950

Application Information

Molecular Weight: Predicted, 20 kDa; observed, 18 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB65

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human MYL12B
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Myosin light chain 12B expression in H9c2 cells using Myosin light chain 12B antibody (Cat#2271, 1:2,000). Green, isotype control; red, Myosin light chain 12B.
  • Immunocytochemical staining of H9C2 cells with anti-Myosin light chain 12B antibody (Cat#2271, 1:1,000). Nuclei were stained blue with DAPI; Myosin light chain 12B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-Myosin light chain 12B antibody (Cat#2271). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Myosin light chain 12B antibody (Cat#2271, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of Myosin light chain 12B expression in H9c2 cells using Myosin light chain 12B antibody (Cat#2271, 1:2,000). Green, isotype control; red, Myosin light chain 12B.
Immunocytochemical staining of H9C2 cells with anti-Myosin light chain 12B antibody (Cat#2271, 1:1,000). Nuclei were stained blue with DAPI; Myosin light chain 12B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Myosin light chain 12B antibody (Cat#2271). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Myosin light chain 12B antibody (Cat#2271, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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