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KD-Validated Anti-Bcl-XL Rabbit Monoclonal Antibody #61349

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Flow cytometric analysis of Bcl-XL expression in HT-1080 cells using Bcl-XL antibody (Cat#61349, 1:2,000). Green, isotype control; red, Bcl-XL.
Immunocytochemical staining of HT-1080 cells with Bcl-XL antibody (Cat#61349, 1:1,000). Nuclei were stained blue with DAPI; Bcl-XL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-Bcl-XL antibody (Cat#61349). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Bcl-XL antibody (Cat#61349, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Bcl-XL antibody (Cat#61349). Bcl-XL expression in wild type (WT) and Bcl-XL shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Bcl-XL antibody (Cat#61349, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Bcl-XL knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Bcl-XL antibody (Cat#61349, 1:2,000) and analyzed using CytoFLEX.
  • 基因名:
    BCL2L1
  • 货号:
    61349
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-Bcl-XL Rabbit Monoclonal Antibody
Aliases BCL2L1; BCL2 Like 1; BCL2L; BCLX; PPP1R52; Protein Phosphatase 1, Regulatory Subunit 52; Apoptosis Regulator Bcl-X; Bcl-2-Like Protein 1; Bcl-X; BCL-XL/S; Bcl2-L-1; Bcl-XL; Bcl-XS; BCL-XL; BCL-XS; BCL-X
Background

Gene Name: BCL2L1

NCBI Gene Entry: 598

UniProt Entry: Q07817

Application Information

Molecular Weight: Predicted, 26 kDa, observed, 26 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3000

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Bcl-XL
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Bcl-XL expression in HT-1080 cells using Bcl-XL antibody (Cat#61349, 1:2,000). Green, isotype control; red, Bcl-XL.
  • Immunocytochemical staining of HT-1080 cells with Bcl-XL antibody (Cat#61349, 1:1,000). Nuclei were stained blue with DAPI; Bcl-XL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
  • Western blotting analysis using anti-Bcl-XL antibody (Cat#61349). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Bcl-XL antibody (Cat#61349, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Bcl-XL antibody (Cat#61349). Bcl-XL expression in wild type (WT) and Bcl-XL shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Bcl-XL antibody (Cat#61349, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of Bcl-XL knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Bcl-XL antibody (Cat#61349, 1:2,000) and analyzed using CytoFLEX.
Citation(0)
Flow cytometric analysis of Bcl-XL expression in HT-1080 cells using Bcl-XL antibody (Cat#61349, 1:2,000). Green, isotype control; red, Bcl-XL.
Immunocytochemical staining of HT-1080 cells with Bcl-XL antibody (Cat#61349, 1:1,000). Nuclei were stained blue with DAPI; Bcl-XL was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-Bcl-XL antibody (Cat#61349). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Bcl-XL antibody (Cat#61349, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Bcl-XL antibody (Cat#61349). Bcl-XL expression in wild type (WT) and Bcl-XL shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Bcl-XL antibody (Cat#61349, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Bcl-XL knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Bcl-XL antibody (Cat#61349, 1:2,000) and analyzed using CytoFLEX.
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