Western blotting analysis using anti-Eukaryotic elongation factor 2 kinase antibody (Cat#62828). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Eukaryotic elongation factor 2 kinase antibody (Cat#62828, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Eukaryotic elongation factor 2 kinase antibody (Cat#62828). Eukaryotic elongation factor 2 kinase expression in wild type (WT) and Eukaryotic elongation factor 2 kinase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Eukaryotic elongation factor 2 kinase antibody (Cat#62828, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:2,500-1:5,000
Note
This product is for research use only.
Picture
Western blotting analysis using anti-Eukaryotic elongation factor 2 kinase antibody (Cat#62828). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Eukaryotic elongation factor 2 kinase antibody (Cat#62828, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Eukaryotic elongation factor 2 kinase antibody (Cat#62828). Eukaryotic elongation factor 2 kinase expression in wild type (WT) and Eukaryotic elongation factor 2 kinase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Eukaryotic elongation factor 2 kinase antibody (Cat#62828, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Eukaryotic elongation factor 2 kinase antibody (Cat#62828). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Eukaryotic elongation factor 2 kinase antibody (Cat#62828, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Eukaryotic elongation factor 2 kinase antibody (Cat#62828). Eukaryotic elongation factor 2 kinase expression in wild type (WT) and Eukaryotic elongation factor 2 kinase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Eukaryotic elongation factor 2 kinase antibody (Cat#62828, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
