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KD-Validated Anti-Caspase 3 p12 Rabbit Monoclonal Antibody #61379

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Flow cytometric analysis of Caspase 3 p12 expression in C2C12 cells using Caspase 3 p12 antibody (Cat#61379, 1:2,000). Green, isotype control; red, Caspase 3 p12.
Immunocytochemical staining of C2C12 cells with Caspase 3 p12 antibody (Cat#61379, 1:1,000). Nuclei were stained blue with DAPI; Caspase 3 p12 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using anti-caspase 3 p12 antibody (Cat#61379, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-Caspase 3 p12 antibody (Cat#61379). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Caspase 3 p12 antibody (Cat#61379, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Caspase 3 p12 antibody (Cat#61379). Total cell lysates (30 μg) from H9c2 and HAP-1 cells untreated or treated with staurosporine (1μM, 3hr) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Caspase 3 p12 antibody (Cat#61379, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-Caspase-3 p12 antibody (Cat#61379). Caspase-3 p12 expression in wild type (WT) and Caspase-3 p12 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Caspase-3 p12 antibody (Cat#61379, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    CASP3
  • 货号：
    61379
  • 应用：
    WB, FCM, IC, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Caspase 3 p12 Rabbit Monoclonal Antibody
Aliases CASP3; Caspase 3; CPP32; Apopain; CPP32B; Caspase 3, Apoptosis-Related Cysteine Peptidase; Caspase 3, Apoptosis-Related Cysteine Protease; SREBP Cleavage Activity 1; Cysteine Protease CPP32; Protein Yama; EC 3.4.22.56; Caspase-3; CASP-3; CPP-32; SCA-1; PARP Cleavage Protease; Procaspase3; EC 3.4.22; APOPAIN; Yama; YAMA
Background

Gene Name: CASP3

NCBI Gene Entry: 836

UniProt Entry: P42574
Application Information

Molecular Weight: Predicted, 32 kDa, observed, 12,29,33 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3310

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC), immunohistochemistry (IHC)
Immunogen A synthesized peptide derived from human Caspase-3 p12
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Immunohistochemistry (IHC): 1:100-1:200
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Caspase 3 p12 expression in C2C12 cells using Caspase 3 p12 antibody (Cat#61379, 1:2,000). Green, isotype control; red, Caspase 3 p12.
  • Immunocytochemical staining of C2C12 cells with Caspase 3 p12 antibody (Cat#61379, 1:1,000). Nuclei were stained blue with DAPI; Caspase 3 p12 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Immunohistochemistry was performed on paraffin-embedded human tonsillitis using anti-caspase 3 p12 antibody (Cat#61379, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Western blotting analysis using anti-Caspase 3 p12 antibody (Cat#61379). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Caspase 3 p12 antibody (Cat#61379, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Caspase 3 p12 antibody (Cat#61379). Total cell lysates (30 μg) from H9c2 and HAP-1 cells untreated or treated with staurosporine (1μM, 3hr) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Caspase 3 p12 antibody (Cat#61379, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-Caspase-3 p12 antibody (Cat#61379). Caspase-3 p12 expression in wild type (WT) and Caspase-3 p12 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Caspase-3 p12 antibody (Cat#61379, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    CASP3
  • 货号：
    61379
  • 应用：
    WB, FCM, IC, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Caspase 3 p12 expression in C2C12 cells using Caspase 3 p12 antibody (Cat#61379, 1:2,000). Green, isotype control; red, Caspase 3 p12.
Immunocytochemical staining of C2C12 cells with Caspase 3 p12 antibody (Cat#61379, 1:1,000). Nuclei were stained blue with DAPI; Caspase 3 p12 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using anti-caspase 3 p12 antibody (Cat#61379, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-Caspase 3 p12 antibody (Cat#61379). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Caspase 3 p12 antibody (Cat#61379, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Caspase 3 p12 antibody (Cat#61379). Total cell lysates (30 μg) from H9c2 and HAP-1 cells untreated or treated with staurosporine (1μM, 3hr) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Caspase 3 p12 antibody (Cat#61379, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-Caspase-3 p12 antibody (Cat#61379). Caspase-3 p12 expression in wild type (WT) and Caspase-3 p12 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Caspase-3 p12 antibody (Cat#61379, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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