Western blotting analysis using anti-HSPA12A antibody (Cat#61420). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HSPA12A antibody (Cat#61420, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-HSPA12A antibody (Cat#61420). HSPA12A expression in wild-type (WT) and HSPA12A knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HSPA12A antibody (Cat#61420, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
HSPA12A; Heat Shock Protein Family A (Hsp70) Member 12A; KIAA0417; Heat Shock 70 KDa Protein 12A; Heat Shock 70kDa Protein 12A; Heat Shock 70kD Protein 12A; FLJ13874
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000
Note
This product is for research use only.
Picture
Western blotting analysis using anti-HSPA12A antibody (Cat#61420). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HSPA12A antibody (Cat#61420, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-HSPA12A antibody (Cat#61420). HSPA12A expression in wild-type (WT) and HSPA12A knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HSPA12A antibody (Cat#61420, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-HSPA12A antibody (Cat#61420). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HSPA12A antibody (Cat#61420, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-HSPA12A antibody (Cat#61420). HSPA12A expression in wild-type (WT) and HSPA12A knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HSPA12A antibody (Cat#61420, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
