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KD-Validated Anti-Glutamate-ammonia ligase Rabbit Monoclonal Antibody #63474

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Flow cytometric analysis of Glutamate-ammonia ligase expression in HeLa cells using Glutamate-ammonia ligase antibody (Cat#63474, 1:2,000). Green, isotype control; red, Glutamate-ammonia ligase.
Immunocytochemical staining of HeLa cells with Glutamate-ammonia ligase antibody (Cat#63474, 1:1,000). Nuclei were stained blue with DAPI; Glutamate-ammonia ligase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Glutamate-ammonia ligase antibody (Cat#63474). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Glutamate-ammonia ligase antibody (Cat#63474, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Glutamate-ammonia ligase antibody (Cat#63474). Glutamate-ammonia ligase expression in wild type (WT) and Glutamate-ammonia ligase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Glutamate-ammonia ligase antibody (Cat#63474, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Glutamine Synthetase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Glutamine Synthetase antibody (Cat#63474, 1:2,000) and analyzed using CytoFLEX.
Immunocytochemical staining of HeLa cells using anti-Glutamate-ammonia ligase antibody (Cat#63474, 1:1,000), Top panel: wild-type (WT); Bottom panal: Glutamate-ammonia ligase shRNA knockdown (KD). Nuclei were stained blue with DAPI; Glutamate-ammonia ligase was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using anti-Glutamate-ammonia ligase antibody (Cat#63474, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • 基因名:
    GLUL
  • 货号:
    63474
  • 应用:
    WB, FCM, IC, IHC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-Glutamate-ammonia ligase Rabbit Monoclonal Antibody
Aliases Glutamate-Ammonia Ligase; Glutamine Synthetase; GLNS; Palmitoyltransferase GLUL; EC 6.3.1.2; GS; Glutamate-Ammonia Ligase (Glutamine Synthase); Cell Proliferation-Inducing Protein; Proliferation-Inducing Protein; Glutamate--Ammonia Ligase; Glutamate Decarboxylase; Glutamine Synthase; EC 2.3.1.225; PIG43; PIG59
Background

Gene Name: GLUL

NCBI Gene Entry: 2752

UniProt Entry: P15104

Application Information

Molecular Weight: Predicted, 42 kDa, observed, 42 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1345

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC), immunohistochemistry (IHC)

Immunogen A synthesized peptide derived from human Glutamine Synthetase
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Immunohistochemistry (IHC): 1:100-1:200
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Glutamate-ammonia ligase expression in HeLa cells using Glutamate-ammonia ligase antibody (Cat#63474, 1:2,000). Green, isotype control; red, Glutamate-ammonia ligase.
  • Immunocytochemical staining of HeLa cells with Glutamate-ammonia ligase antibody (Cat#63474, 1:1,000). Nuclei were stained blue with DAPI; Glutamate-ammonia ligase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Glutamate-ammonia ligase antibody (Cat#63474). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Glutamate-ammonia ligase antibody (Cat#63474, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Glutamate-ammonia ligase antibody (Cat#63474). Glutamate-ammonia ligase expression in wild type (WT) and Glutamate-ammonia ligase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Glutamate-ammonia ligase antibody (Cat#63474, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of Glutamine Synthetase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Glutamine Synthetase antibody (Cat#63474, 1:2,000) and analyzed using CytoFLEX.
  • Immunocytochemical staining of HeLa cells using anti-Glutamate-ammonia ligase antibody (Cat#63474, 1:1,000), Top panel: wild-type (WT); Bottom panal: Glutamate-ammonia ligase shRNA knockdown (KD). Nuclei were stained blue with DAPI; Glutamate-ammonia ligase was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using anti-Glutamate-ammonia ligase antibody (Cat#63474, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Citation(0)
Flow cytometric analysis of Glutamate-ammonia ligase expression in HeLa cells using Glutamate-ammonia ligase antibody (Cat#63474, 1:2,000). Green, isotype control; red, Glutamate-ammonia ligase.
Immunocytochemical staining of HeLa cells with Glutamate-ammonia ligase antibody (Cat#63474, 1:1,000). Nuclei were stained blue with DAPI; Glutamate-ammonia ligase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Glutamate-ammonia ligase antibody (Cat#63474). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Glutamate-ammonia ligase antibody (Cat#63474, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Glutamate-ammonia ligase antibody (Cat#63474). Glutamate-ammonia ligase expression in wild type (WT) and Glutamate-ammonia ligase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Glutamate-ammonia ligase antibody (Cat#63474, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Glutamine Synthetase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Glutamine Synthetase antibody (Cat#63474, 1:2,000) and analyzed using CytoFLEX.
Immunocytochemical staining of HeLa cells using anti-Glutamate-ammonia ligase antibody (Cat#63474, 1:1,000), Top panel: wild-type (WT); Bottom panal: Glutamate-ammonia ligase shRNA knockdown (KD). Nuclei were stained blue with DAPI; Glutamate-ammonia ligase was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using anti-Glutamate-ammonia ligase antibody (Cat#63474, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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