首页

关于我们

善本标准

产品中心

定制服务

信号通路

促销活动

技术支持

新闻资讯

  • 一抗
  • 二抗
  • 慢病毒
  • 细胞系
  • 裂解物
  • 试剂类
首页>产品中心>一抗

KD-Validated Anti-Hydroxymethylbilane synthase Rabbit Monoclonal Antibody #61422

一键复制产品信息

返回列表

Flow cytometric analysis of Hydroxymethylbilane synthase expression in HeLa cells usiHydroxymethylbilane synthase0 α antibody (Cat#61422, 1:2,000). Green, isotype control; red, Hydroxymethylbilane synthase.
Immunocytochemical staining of HeLa cells with anti-Hydroxymethylbilane synthase antibody (Cat#61422, 1:1,000). Nuclei were stained blue with DAPI; Hydroxymethylbilane synthase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-shydroxymethylbilane synthase antibody (Cat#61422). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-shydroxymethylbilane synthase antibody (Cat#61422, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-hydroxymethylbilane synthase antibody (Cat#61422). Hydroxymethylbilane synthase expression in wild-type (WT) and hydroxymethylbilane synthase (HMBS) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-hydroxymethylbilane synthase antibody (Cat#61422, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Hydroxymethylbilane synthase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Hydroxymethylbilane synthase antibody (Cat#61422, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Hydroxymethylbilane synthase antibody (Cat#61422, 1:1,000), Top panel: wild-type (WT); Bottom panal: Hydroxymethylbilane synthase shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • 基因名：
    HMBS
  • 货号：
    61422
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Hydroxymethylbilane synthase Rabbit Monoclonal Antibody
Aliases HMBS; Hydroxymethylbilane Synthase; Porphobilinogen Deaminase; PBGD; UPS; Porphyria, Acute; Chester Type; Pre-Uroporphyrinogen Synthase; Uroporphyrinogen I Synthase; EC 2.5.1.61; PBG-D; PORC; Uroporphyrinogen I Synthetase
Background

Gene Name: HMBS

NCBI Gene Entry: 3145

UniProt Entry: P08397
Application Information

Molecular Weight: Predicted, 39 kDa; observed, 42 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2490

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human HMBS
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Hydroxymethylbilane synthase expression in HeLa cells usiHydroxymethylbilane synthase0 α antibody (Cat#61422, 1:2,000). Green, isotype control; red, Hydroxymethylbilane synthase.
  • Immunocytochemical staining of HeLa cells with anti-Hydroxymethylbilane synthase antibody (Cat#61422, 1:1,000). Nuclei were stained blue with DAPI; Hydroxymethylbilane synthase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-shydroxymethylbilane synthase antibody (Cat#61422). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-shydroxymethylbilane synthase antibody (Cat#61422, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-hydroxymethylbilane synthase antibody (Cat#61422). Hydroxymethylbilane synthase expression in wild-type (WT) and hydroxymethylbilane synthase (HMBS) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-hydroxymethylbilane synthase antibody (Cat#61422, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of Hydroxymethylbilane synthase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Hydroxymethylbilane synthase antibody (Cat#61422, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HeLa cells using anti-Hydroxymethylbilane synthase antibody (Cat#61422, 1:1,000), Top panel: wild-type (WT); Bottom panal: Hydroxymethylbilane synthase shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Citation(0)
  • 基因名：
    HMBS
  • 货号：
    61422
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Hydroxymethylbilane synthase expression in HeLa cells usiHydroxymethylbilane synthase0 α antibody (Cat#61422, 1:2,000). Green, isotype control; red, Hydroxymethylbilane synthase.
Immunocytochemical staining of HeLa cells with anti-Hydroxymethylbilane synthase antibody (Cat#61422, 1:1,000). Nuclei were stained blue with DAPI; Hydroxymethylbilane synthase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-shydroxymethylbilane synthase antibody (Cat#61422). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-shydroxymethylbilane synthase antibody (Cat#61422, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-hydroxymethylbilane synthase antibody (Cat#61422). Hydroxymethylbilane synthase expression in wild-type (WT) and hydroxymethylbilane synthase (HMBS) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-hydroxymethylbilane synthase antibody (Cat#61422, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Hydroxymethylbilane synthase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Hydroxymethylbilane synthase antibody (Cat#61422, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Hydroxymethylbilane synthase antibody (Cat#61422, 1:1,000), Top panel: wild-type (WT); Bottom panal: Hydroxymethylbilane synthase shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • 联系电话

    0551-63635518

    0551-65578998

  • 联系邮箱

    support@genuinbiotech.cn

    sales@genuinbiotech.cn

微信公众号

Copyright © 2025 合肥善本生物科技有限公司

All Rights Reserved. 皖ICP备2023000814号