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KD-Validated Anti-Phospho-c-Myc (S62) Rabbit Monoclonal Antibody #61451

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Flow cytometric analysis of Phospho-c-Myc (S62) expression in HeLa cells using Phospho-c-Myc (S62) antibody (Cat#61451, 1:2,000). Green, isotype control; red, Phospho-c-Myc (S62).
Immunocytochemical staining of HeLa cells with anti-Phospho-c-Myc (S62) antibody (Cat#61451, 1:1,000). Nuclei were stained blue with DAPI; Phospho-c-Myc (S62) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-c-Myc (S62) antibody (Cat#61451). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-c-Myc (S62) antibody (Cat#61451, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-phospho-c-Myc (S62) antibody (Cat#61451). Phospho-c-Myc (S62) expression in wild type (WT) and MYC knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-c-Myc (S62) antibody (Cat#61451, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    MYC
  • 货号：
    61451
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Phospho-c-Myc (S62) Rabbit Monoclonal Antibody
Aliases MYC Proto-Oncogene, BHLH Transcription Factor; BHLHe39; C-Myc; MYCC; V-Myc Avian Myelocytomatosis Viral Oncogene Homolog; Class E Basic Helix-Loop-Helix Protein 39; Myc Proto-Oncogene Protein; Transcription Factor P64; Proto-Oncogene C-Myc; Myc-Related Translation/Localization Regulatory Factor; Avian Myelocytomatosis Viral Oncogene Homolog; V-Myc Myelocytomatosis Viral Oncogene Homolog; BHLHE39; MRTL
Background

Gene Name: MYC

NCBI Gene Entry: 4609

UniProt Entry: P01106
Application Information

Molecular Weight: Predicted, 51 kDa, observed, 57 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2565

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Phospho-c-Myc (S62)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Phospho-c-Myc (S62) expression in HeLa cells using Phospho-c-Myc (S62) antibody (Cat#61451, 1:2,000). Green, isotype control; red, Phospho-c-Myc (S62).
  • Immunocytochemical staining of HeLa cells with anti-Phospho-c-Myc (S62) antibody (Cat#61451, 1:1,000). Nuclei were stained blue with DAPI; Phospho-c-Myc (S62) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-phospho-c-Myc (S62) antibody (Cat#61451). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-c-Myc (S62) antibody (Cat#61451, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-phospho-c-Myc (S62) antibody (Cat#61451). Phospho-c-Myc (S62) expression in wild type (WT) and MYC knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-c-Myc (S62) antibody (Cat#61451, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    MYC
  • 货号：
    61451
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Phospho-c-Myc (S62) expression in HeLa cells using Phospho-c-Myc (S62) antibody (Cat#61451, 1:2,000). Green, isotype control; red, Phospho-c-Myc (S62).
Immunocytochemical staining of HeLa cells with anti-Phospho-c-Myc (S62) antibody (Cat#61451, 1:1,000). Nuclei were stained blue with DAPI; Phospho-c-Myc (S62) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-c-Myc (S62) antibody (Cat#61451). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-c-Myc (S62) antibody (Cat#61451, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-phospho-c-Myc (S62) antibody (Cat#61451). Phospho-c-Myc (S62) expression in wild type (WT) and MYC knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-c-Myc (S62) antibody (Cat#61451, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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