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KD-Validated Anti-NFKB1 Rabbit Monoclonal Antibody #61524

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Flow cytometric analysis of NFKB1 expression in C2C12 cells using NFKB1 antibody (Cat#61524, 1:2,000). Green, isotype control; red, NFKB1.
Immunocytochemical staining of C2C12 cells with NFKB1 antibody (Cat#61524, 1:1,000). Nuclei were stained blue with DAPI; NFKB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-NFKB1 antibody (Cat#61524). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NFKB1 antibody (Cat#61524, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NFKB1 antibody (Cat#61524). NFKB1 expression in wild type (WT) and NFKB1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NFKB1 antibody (Cat#61524, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    NFKB1
  • 货号：
    61524
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-NFKB1 Rabbit Monoclonal Antibody
Aliases NFKB1; Nuclear Factor Kappa B Subunit 1; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear Factor NF-Kappa-B P105 Subunit; NFKB-P50; NF-KB1; KBF1; DNA-Binding Factor KBF1; NF-KappaB; NFkappaB; EBP-1; P105; P50; Nuclear Factor Kappa-B DNA Binding Subunit; Nuclear Factor NF-Kappa-B P50 Subunit; NF-Kappabeta; NF-Kappa-B1; NFKB-P105; NF-KAPPAB; NFKAPPAB; CVID12; NF-KB
Background

Gene Name: NFKB1

NCBI Gene Entry: 4790

UniProt Entry: P19838
Application Information

Molecular Weight: Predicted, 105 kDa, observed, 50,105 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2710

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human NF-κB (p105/p50)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of NFKB1 expression in C2C12 cells using NFKB1 antibody (Cat#61524, 1:2,000). Green, isotype control; red, NFKB1.
  • Immunocytochemical staining of C2C12 cells with NFKB1 antibody (Cat#61524, 1:1,000). Nuclei were stained blue with DAPI; NFKB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-NFKB1 antibody (Cat#61524). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NFKB1 antibody (Cat#61524, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-NFKB1 antibody (Cat#61524). NFKB1 expression in wild type (WT) and NFKB1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NFKB1 antibody (Cat#61524, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    NFKB1
  • 货号：
    61524
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of NFKB1 expression in C2C12 cells using NFKB1 antibody (Cat#61524, 1:2,000). Green, isotype control; red, NFKB1.
Immunocytochemical staining of C2C12 cells with NFKB1 antibody (Cat#61524, 1:1,000). Nuclei were stained blue with DAPI; NFKB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-NFKB1 antibody (Cat#61524). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NFKB1 antibody (Cat#61524, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-NFKB1 antibody (Cat#61524). NFKB1 expression in wild type (WT) and NFKB1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NFKB1 antibody (Cat#61524, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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