Flow cytometric analysis of GAPDH expression in HepG2 cells using anti-GAPDH antibody (Cat#61536, 1:2,000). Green, isotype control; red, GAPDH.
Immunocytochemical staining of HepG2 cells with anti-GAPDH antibody (Cat#61536, 1:1,000). Nuclei were stained blue with DAPI; GAPDH was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-GAPDH antibody (Cat#61536). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GAPDH antibody (Cat#61536, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-GAPDH antibody (Cat#61536). GAPDH expression in wild type (WT) and GAPDH knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GAPDH antibody (Cat#61536, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
