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KD-Validated Anti-AP2M1 Rabbit Monoclonal Antibody #61579

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Immunocytochemical staining of C2C12 cells with AP2M1 antibody (Cat#61579, 1:1,000). Nuclei were stained blue with DAPI; AP2M1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-AP2M1 antibody (Cat#61579). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AP2M1 antibody (Cat#61579, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-AP2M1 antibody (Cat#61579). AP2M1 expression in wild type (WT) and AP2M1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-AP2M1 antibody (Cat#61579, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    AP2M1
  • 货号：
    61579
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-AP2M1 Rabbit Monoclonal Antibody
Aliases AP2M1; Adaptor Related Protein Complex 2 Subunit Mu 1; CLAPM1; AP50; Clathrin-Associated/Assembly/Adaptor Protein, Medium 1; Clathrin Assembly Protein Complex 2 Mu Medium Chain; Clathrin Assembly Protein Complex 2 Medium Chain; Adaptor Related Protein Complex 2 Mu 1 Subunit; Adaptor-Related Protein Complex 2 Subunit Mu; Plasma Membrane Adaptor AP-2 50 KDa Protein; Plasma Membrane Adaptor AP-2 50kDA Protein; Clathrin Adaptor Complex AP2, Mu Subunit; Adaptor Protein Complex AP-2 Subunit Mu; Clathrin Coat-Associated Protein AP50; Clathrin Coat Assembly Protein AP50; Clathrin Coat Adaptor Protein AP50; AP-2 Complex Subunit Mu; HA2 50 KDA Subunit; AP-2 Mu 2 Chain; Adaptin-Mu2; Mu2; HA2 50 KDa Subunit; AP-2 Mu Chain; KIAA0109; MRD60; MU2
Background

Gene Name: AP2M1

NCBI Gene Entry: 1173

UniProt Entry: Q96CW1
Application Information

Molecular Weight: Predicted, 50 kDa, observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3220

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human AP2M1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of C2C12 cells with AP2M1 antibody (Cat#61579, 1:1,000). Nuclei were stained blue with DAPI; AP2M1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
  • Western blotting analysis using anti-AP2M1 antibody (Cat#61579). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AP2M1 antibody (Cat#61579, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-AP2M1 antibody (Cat#61579). AP2M1 expression in wild type (WT) and AP2M1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-AP2M1 antibody (Cat#61579, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    AP2M1
  • 货号：
    61579
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Immunocytochemical staining of C2C12 cells with AP2M1 antibody (Cat#61579, 1:1,000). Nuclei were stained blue with DAPI; AP2M1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-AP2M1 antibody (Cat#61579). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AP2M1 antibody (Cat#61579, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-AP2M1 antibody (Cat#61579). AP2M1 expression in wild type (WT) and AP2M1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-AP2M1 antibody (Cat#61579, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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