Flow cytometric analysis of NEDD4L expression in HepG2 cells using anti-NEDD4L antibody (Cat#61581, 1:2,000). Green, isotype control; red, NEDD4L.
lmmunocytochemical staining of HepG2 cells with NEDD4L antibody (Cat#61581, 1:1,000). Nuclei were stained blue with DAPl; NEDD4L was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NEDD4L antibody (Cat#61581). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NEDD4L antibody (Cat#61581, 1:20,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-NEDD4L antibody (Cat#61581). NEDD4L expression in wild-type (WT) and NEDD4L knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NEDD4L antibody (Cat#61581, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
