Flow cytometric analysis of ATG4B expression in C2C12 cells using ATG4B antibody (Cat#61584, 1:2000). Green, isotype control; red, ATG4B.
Immunocytochemical staining of C2C12 cells with ATG4B antibody (Cat#61584, 1:1,000). Nuclei were stained blue with DAPI; ATG4B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-ATG4B antibody (Cat#61584). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATG4B antibody (Cat#61584, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-ATG4B antibody (Cat#61584). ATG4B expression in wild type (WT) and ATG4B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ATG4B antibody (Cat#61584, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of ATG4B knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-ATG4B antibody (Cat#61584, 1:2,000) and analyzed using BD flow cytometer.
