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KD-Validated Anti-Caspase 6 Rabbit Monoclonal Antibody #62027

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Immunocytochemical staining of C2C12 cells with anti-Caspase 6 antibody (Cat#62027, 1:1,000). Nuclei were stained blue with DAPI; Caspase 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.

Western blotting analysis using anti-Caspase 6 antibody (Cat#62027). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Caspase 6 antibody (Cat#62027, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-caspase 6 antibody (Cat#62027). Caspase 6 expression in wild-type (WT) and caspase 6 (CASP6) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-caspase 6 antibody (Cat#62027, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

CASP6
货号：

62027
应用：

WB, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-Caspase 6 Rabbit Monoclonal Antibody
Aliases	CASP6; Caspase 6; CSP-6; MCH2; Caspase-6; Caspase 6, Apoptosis-Related Cysteine Peptidase; Caspase 6, Apoptosis-Related Cysteine Protease; Mammalian Ced-3 Homologue 2; EC 3.4.22.59; Apoptotic Protease MCH-2; Apoptotic Protease Mch-2; CASPASE-6; EC 3.4.22; CASP-6
Background	Gene Name: CASP6 NCBI Gene Entry: 839 UniProt Entry: P55212
Application Information	Molecular Weight: Predicted, 33 kDa, observed, 33 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB5635 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Caspase-6
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Immunocytochemical staining of C2C12 cells with anti-Caspase 6 antibody (Cat#62027, 1:1,000). Nuclei were stained blue with DAPI; Caspase 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-Caspase 6 antibody (Cat#62027). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Caspase 6 antibody (Cat#62027, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-caspase 6 antibody (Cat#62027). Caspase 6 expression in wild-type (WT) and caspase 6 (CASP6) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-caspase 6 antibody (Cat#62027, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

CASP6
货号：

62027
应用：

WB, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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