Flow cytometric analysis of PARP expression in HAP-1 cells using PARP antibody (Cat#1290, 1:2,000). Green, isotype control; red, PARP.
Immunocytochemical staining of HAP-1 cells with PARP antibody (Cat#1290, 1:1,000). Nuclei were stained blue with DAPI; PARP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-PARP antibody (Cat#1290). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PARP antibody (Cat#1290, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-PARP antibody (Cat#1290). Total cell lysates (30 μg) from HeLa and HAP-1 cells untreated or treated with staurosporine (1μM, 3hr) were loaded and separated by SDS-PAGE. The blot was incubated with anti-PARP antibody (Cat#1290, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).