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KD-Validated Anti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody #61644

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Flow cytometric analysis of Phospho-EGFR (Y1173) expression in HepG2 cells using Phospho-EGFR (Y1173) antibody (Cat#61644, 1:2,000). Green, isotype control; red, Phospho-EGFR (Y1173).
Immunocytochemical staining of HepG2 cells with anti-Phospho-EGFR (Y1173) antibody (Cat #61644, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-EGFR (Y1173) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Phospho-EGFR (Y1173) antibody (Cat#61644). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-EGFR (Y1173) antibody (Cat#61644, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-EGFR (Y1173) antibody (Cat#61644). Phospho-EGFR (Y1173) expression in wild type (WT) and Phospho-EGFR (Y1173) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Phospho-EGFR (Y1173) antibody (Cat#61644, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    EGFR
  • 货号：
    61644
  • 应用：
    WB, IC, FCM
  • 物种反应性：
    H, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Phospho-EGFR (Y1173) Rabbit Monoclonal Antibody
Aliases EGFR; Epidermal Growth Factor Receptor; ERBB1; ERRP; ERBB; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian); Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog; Epidermal Growth Factor Receptor Tyrosine Kinase Domain; Cell Proliferation-Inducing Protein 61; Cell Growth Inhibiting Protein 40; EGFR VIII; EC 2.7.10; NISBD2; PIG61; MENA
Background

Gene Name: EGFR

NCBI Gene Entry: 1956

UniProt Entry: P00533
Application Information

Molecular Weight: Predicted, 134 kDa, observed, 175 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3900

Species Reactivity: Human, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Phospho-EGFR (Y1173)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Phospho-EGFR (Y1173) expression in HepG2 cells using Phospho-EGFR (Y1173) antibody (Cat#61644, 1:2,000). Green, isotype control; red, Phospho-EGFR (Y1173).
  • Immunocytochemical staining of HepG2 cells with anti-Phospho-EGFR (Y1173) antibody (Cat #61644, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-EGFR (Y1173) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-Phospho-EGFR (Y1173) antibody (Cat#61644). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-EGFR (Y1173) antibody (Cat#61644, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Phospho-EGFR (Y1173) antibody (Cat#61644). Phospho-EGFR (Y1173) expression in wild type (WT) and Phospho-EGFR (Y1173) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Phospho-EGFR (Y1173) antibody (Cat#61644, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    EGFR
  • 货号：
    61644
  • 应用：
    WB, IC, FCM
  • 物种反应性：
    H, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Phospho-EGFR (Y1173) expression in HepG2 cells using Phospho-EGFR (Y1173) antibody (Cat#61644, 1:2,000). Green, isotype control; red, Phospho-EGFR (Y1173).
Immunocytochemical staining of HepG2 cells with anti-Phospho-EGFR (Y1173) antibody (Cat #61644, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-EGFR (Y1173) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Phospho-EGFR (Y1173) antibody (Cat#61644). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-EGFR (Y1173) antibody (Cat#61644, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-EGFR (Y1173) antibody (Cat#61644). Phospho-EGFR (Y1173) expression in wild type (WT) and Phospho-EGFR (Y1173) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Phospho-EGFR (Y1173) antibody (Cat#61644, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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