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KD-Validated Anti-Phospho-eIF4E (S209) Rabbit Monoclonal Antibody #61666

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Flow cytometric analysis of Phospho-eIF4E (S209) expression in C2C12 cells using anti-Phospho-eIF4E (S209) antibody (Cat#61666, 1:2,000). Green, isotype control; red, Phospho-eIF4E (S209).
Immunocytochemical staining of C2C12 cells with Phospho-eIF4E (S209) antibody (Cat#61666, 1:1,000). Nuclei were stained blue with DAPI; Phospho-eIF4E (S209) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-eIF4E (S209) antibody (Cat#61666). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-eIF4E (S209) antibody (Cat#61666, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-phospho-eIF4E (S209) antibody (Cat#61666). Phospho-eIF4E (S209) expression in wild type (WT) and EIF4E knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-eIF4E (S209) antibody (Cat#61666, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    EIF4E
  • 货号：
    61666
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Phospho-eIF4E (S209) Rabbit Monoclonal Antibody
Aliases EIF4E; Eukaryotic Translation Initiation Factor 4E; EIF4EL1; EIF4E1; EIF4F; MRNA Cap-Binding Protein; EIF-4F 25 KDa Subunit; EIF-4E; Eukaryotic Translation Initiation Factor 4E-Like 1; AUTS19; EIF4E; CBP
Background

Gene Name: EIF4E

NCBI Gene Entry: 1977

UniProt Entry: P06730
Application Information

Molecular Weight: Predicted, 25 kDa, observed, 25 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB3235

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Phospho-eIF4E (S209)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Phospho-eIF4E (S209) expression in C2C12 cells using anti-Phospho-eIF4E (S209) antibody (Cat#61666, 1:2,000). Green, isotype control; red, Phospho-eIF4E (S209).
  • Immunocytochemical staining of C2C12 cells with Phospho-eIF4E (S209) antibody (Cat#61666, 1:1,000). Nuclei were stained blue with DAPI; Phospho-eIF4E (S209) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Western blotting analysis using anti-phospho-eIF4E (S209) antibody (Cat#61666). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-eIF4E (S209) antibody (Cat#61666, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-phospho-eIF4E (S209) antibody (Cat#61666). Phospho-eIF4E (S209) expression in wild type (WT) and EIF4E knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-eIF4E (S209) antibody (Cat#61666, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    EIF4E
  • 货号：
    61666
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Phospho-eIF4E (S209) expression in C2C12 cells using anti-Phospho-eIF4E (S209) antibody (Cat#61666, 1:2,000). Green, isotype control; red, Phospho-eIF4E (S209).
Immunocytochemical staining of C2C12 cells with Phospho-eIF4E (S209) antibody (Cat#61666, 1:1,000). Nuclei were stained blue with DAPI; Phospho-eIF4E (S209) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-eIF4E (S209) antibody (Cat#61666). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-eIF4E (S209) antibody (Cat#61666, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-phospho-eIF4E (S209) antibody (Cat#61666). Phospho-eIF4E (S209) expression in wild type (WT) and EIF4E knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-eIF4E (S209) antibody (Cat#61666, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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