KD-Validated Anti-POLD1 Rabbit Monoclonal Antibody - 产品中心

KD-Validated Anti-POLD1 Rabbit Monoclonal Antibody #61690

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Flow cytometric analysis of POLD1 expression in HepG2 cells using anti-POLD1 antibody (Cat#61690, 1:2,000). Green, isotype control; red, POLD1.

Immunocytochemical staining of HAP-1 cells with anti-POLD1 antibody (Cat#61690, 1:1,000). Nuclei were stained blue with DAPI; POLD1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-POLD1 antibody (Cat#61690). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-POLD1 antibody (Cat#61690, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-POLD1 antibody (Cat#61690). POLD1 expression in wild-type (WT) and POLD1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-POLD1 antibody (Cat#61690, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

POLD1
货号：

61690
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-POLD1 Rabbit Monoclonal Antibody
Aliases	POLD1; DNA Polymerase Delta 1, Catalytic Subunit; CDC2; POLD; Polymerase (DNA) Delta 1, Catalytic Subunit; DNA Polymerase Delta Catalytic Subunit; DNA Polymerase Subunit Delta P125; 3'-5' Exodeoxyribonuclease; EC 2.7.7.7; Polymerase (DNA Directed), Delta 1, Catalytic Subunit (125kD); Polymerase (DNA Directed), Delta 1, Catalytic Subunit 125kDa; CDC2 Homolog (S. Cerevisiae); CDC2 Homolog; EC 3.1.11.-; CRCS10; MDPL
Background	Gene Name: POLD1 NCBI Gene Entry: 5424 UniProt Entry: P28340
Application Information	Molecular Weight: Predicted, 124 kDa; observed, 124 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB3335 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human POLD1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of POLD1 expression in HepG2 cells using anti-POLD1 antibody (Cat#61690, 1:2,000). Green, isotype control; red, POLD1.
Immunocytochemical staining of HAP-1 cells with anti-POLD1 antibody (Cat#61690, 1:1,000). Nuclei were stained blue with DAPI; POLD1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-POLD1 antibody (Cat#61690). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-POLD1 antibody (Cat#61690, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-POLD1 antibody (Cat#61690). POLD1 expression in wild-type (WT) and POLD1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-POLD1 antibody (Cat#61690, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

POLD1
货号：

61690
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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