Flow cytometric analysis of ATP synthase F1 subunit gamma expression in HepG2 cells using anti-ATP synthase F1 subunit gamma antibody (Cat#63811, 1:1,000). Green, isotype control; red, ATP synthase F1 subunit gamma.
Immunocytochemical staining of HepG2 cells with anti-ATP synthase F1 subunit gamma antibody (Cat#63811, 1:500) . Nuclei were stained blue with DAPI; ATP synthase F1 subunit gamma was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-ATP synthase F1 subunit gamma antibody (Cat#63811). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP synthase F1 subunit gamma antibody (Cat#63811, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-ATP synthase F1 subunit gamma antibody (Cat#63811). ATP synthase F1 subunit gamma expression in wild type (WT) and ATP synthase F1 subunit gamma (ATP5F1C) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ATP synthase F1 subunit gamma antibody (Cat#63811, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
